Factors affecting the mixed leucocyte culture as histocompatibility test: II. comparative studies with granulocytes, lymphocytes and macrophages

G. A. Nagel, T. J. Matulewski, J. F. Holland

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The mitogenic activities of various leucocyte preparations in human short term mixed leucocyte cultures (MLC) were compared. Thus the degree of mitogenicity is different for each cell type in the order: granulocytes, lymphocytes, macrophages. Mitomycin C treatment drastically reduced the mitogenicity of granulocytes. Lymphocyte transformation induced by Rebuck skin window macrophages was extremely weak when rather purified allogeneic macrophages were used as stimulants. Macrophages were, however, very potent mitogens, probably mediating specific immunologic information, when added supplementary to granulocytes. It is suggested that the MLC can be improved as a histocompatibility test by standardizing the stimulatory cell pool with respect to its qualitative and quantitative cell compositions.

Original languageEnglish
Pages (from-to)370-379
Number of pages10
JournalEuropean Surgical Research
Volume2
Issue number5
DOIs
StatePublished - 1970
Externally publishedYes

Keywords

  • Granulocytes
  • Histocompatibility
  • Lymphocytes
  • Macrophages

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