TY - JOUR
T1 - Factors affecting antigen uptake by human intestinal epithelial cell lines
AU - So, Agnes Laiping
AU - Small, Gillian
AU - Sperber, Kirk
AU - Becker, Kai
AU - Oei, Erwin
AU - Tyorkin, Max
AU - Mayer, Lloyd
N1 - Funding Information:
Supported by PHS grants AI 23504, AI 24671 (L.M.), AHA92008500 (G.S.) and R29CA66522-3 (K.S.) and by the Kennedy-Leigh Trust (L.M.) and the Irma T. Hirschl Career Development Trust (K.S.).
PY - 2000
Y1 - 2000
N2 - We assessed the role of size, solubility, and prophagocytic cytokines interferon-γ (IFN-γ), and granulocyte-macrophage colony Stimulatory factor (GM-CSF) in antigen uptake and kinetics by intestinal epithelial cells using keyhole limpet hemocyanin and ovalbumin. Both fluoresceinated keyhole limpet hemocyanin (3000-7500 kDa) and fluoresceinated ovalbumin (45 kDa) were internalized by human colonic epithelial cell lines, with kinetics similar to those of fluoresceinated tetanus toxoid, and there was decreased uptake of insoluble immune complexes and no enhancement in the uptake of soluble immune complexes. In addition, neither IFN-γ nor GM-CSF altered the kinetics of uptake nor enhanced antigen internalization by the intestinal epithelial cell lines. These data suggest that regardless of the size of the soluble antigen, the presence of prophagocytic cytokines, or the formation of soluble immune complexes, fluid phase endocytosis of antigen by intestinal epithelial cells appears to be a relatively stable process.
AB - We assessed the role of size, solubility, and prophagocytic cytokines interferon-γ (IFN-γ), and granulocyte-macrophage colony Stimulatory factor (GM-CSF) in antigen uptake and kinetics by intestinal epithelial cells using keyhole limpet hemocyanin and ovalbumin. Both fluoresceinated keyhole limpet hemocyanin (3000-7500 kDa) and fluoresceinated ovalbumin (45 kDa) were internalized by human colonic epithelial cell lines, with kinetics similar to those of fluoresceinated tetanus toxoid, and there was decreased uptake of insoluble immune complexes and no enhancement in the uptake of soluble immune complexes. In addition, neither IFN-γ nor GM-CSF altered the kinetics of uptake nor enhanced antigen internalization by the intestinal epithelial cell lines. These data suggest that regardless of the size of the soluble antigen, the presence of prophagocytic cytokines, or the formation of soluble immune complexes, fluid phase endocytosis of antigen by intestinal epithelial cells appears to be a relatively stable process.
KW - Antigen uptake
KW - Cytokines
KW - Immune complexes
UR - http://www.scopus.com/inward/record.url?scp=0034097963&partnerID=8YFLogxK
U2 - 10.1023/A:1005593717644
DO - 10.1023/A:1005593717644
M3 - Article
C2 - 10877228
AN - SCOPUS:0034097963
SN - 0163-2116
VL - 45
SP - 1130
EP - 1137
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 6
ER -