TY - JOUR
T1 - Eya1 and Eya2 proteins are required for hypaxial somitic myogenesis in the mouse embryo
AU - Grifone, Raphaelle
AU - Demignon, Josiane
AU - Giordani, Julien
AU - Niro, Claire
AU - Souil, Evelyne
AU - Bertin, Florence
AU - Laclef, Christine
AU - Xu, Pin Xian
AU - Maire, Pascal
N1 - Funding Information:
We thank F. Relaix and M. Lagha for their helpful support concerning in situ analysis, and for helpful critical reading of the manuscript. We thank S. Gautron and F. Francis for helpful corrections of the manuscript. We thank S. Tajbakhsh for the gift of Pax3−/+ mice, and L. Renou and D. Touati for helpful technical assistance. R.G was supported by a fellowship from the Ministère de la Recherche et de l'Education nationale, from the “Association Française contre les Myopathies” (AFM) and from the Fondation pour la Recherche Médicale (FRM). Financial support for this work has been provided by NIH RO1-DC05824, RO1-DK64640 and Oberkotter foundation to P-X. Xu, and by the Institut National pour la Santé et la Recherche Médicale (INSERM), by the AFM, by the Centre National de la Recherche Scientifique (CNRS), by the Action Concertée Incitative 0220514, by the Association pour la Recherche sur le Cancer (ARC) and by the FP6 Myores european network to P.Maire. The contribution of the Région Ile de France to the Institut Cochin animal care facility is also acknowledged.
PY - 2007/2/15
Y1 - 2007/2/15
N2 - In mammals, Pax3, Six4, Six1 and Six5 genes are co-expressed with Eya1, Eya2 and Eya4 genes during mouse somitogenesis. To unravel the functions of Eya genes during muscle development, we analyzed myogenesis in Eya2-/- and in Eya1-/- embryos. A delay in limb myogenesis was observed between E10 and E13 in Eya1-/- embryos only, that is later compensated. Compound E18 Eya1-/-Eya2-/+ fetuses present a muscle phenotype comparable with that of Six1-/- fetuses; lacking a diaphragm and with a specific absence of limb muscles, suggesting either genetic epistasis between Six and Eya genes, or biochemical interactions between Six and Eya proteins. We tested these two non-exclusive possibilities. First, we show that Six proteins recruit Eya proteins to drive transcription during embryogenesis in the dermomyotomal epaxial and hypaxial lips of the somites by binding MEF3 DNA sites. Second, we show that Pax3 expression is lost in the ventrolateral (hypaxial) dermomyotomes of the somite in both Eya1-/-Eya2-/- embryos and in Six1-/-Six4-/- embryos, precluding hypaxial lip formation. This structure, from which myogenic cells delaminate to invade the limb does not form in these double mutant embryos, leading to limb buds without myogenic progenitor cells. Eya1 and Eya2, however, are still expressed in the somites of Six1Six4 double mutant and in splotch embryos, and Six1 is expressed in the somites of Eya1Eya2 double mutant embryos and in splotch embryos. Altogether these results show that Six and Eya genes lie genetically upstream of Pax3 gene in the formation of ventrolateral dermomyotome hypaxial lips. No genetic links have been characterized between Six and Eya genes, but corresponding proteins activate key muscle determination genes (Myod, Myogenin and Mrf4). These results establish a new hierarchy of genes controlling early steps of hypaxial myogenic commitment in the mouse embryo.
AB - In mammals, Pax3, Six4, Six1 and Six5 genes are co-expressed with Eya1, Eya2 and Eya4 genes during mouse somitogenesis. To unravel the functions of Eya genes during muscle development, we analyzed myogenesis in Eya2-/- and in Eya1-/- embryos. A delay in limb myogenesis was observed between E10 and E13 in Eya1-/- embryos only, that is later compensated. Compound E18 Eya1-/-Eya2-/+ fetuses present a muscle phenotype comparable with that of Six1-/- fetuses; lacking a diaphragm and with a specific absence of limb muscles, suggesting either genetic epistasis between Six and Eya genes, or biochemical interactions between Six and Eya proteins. We tested these two non-exclusive possibilities. First, we show that Six proteins recruit Eya proteins to drive transcription during embryogenesis in the dermomyotomal epaxial and hypaxial lips of the somites by binding MEF3 DNA sites. Second, we show that Pax3 expression is lost in the ventrolateral (hypaxial) dermomyotomes of the somite in both Eya1-/-Eya2-/- embryos and in Six1-/-Six4-/- embryos, precluding hypaxial lip formation. This structure, from which myogenic cells delaminate to invade the limb does not form in these double mutant embryos, leading to limb buds without myogenic progenitor cells. Eya1 and Eya2, however, are still expressed in the somites of Six1Six4 double mutant and in splotch embryos, and Six1 is expressed in the somites of Eya1Eya2 double mutant embryos and in splotch embryos. Altogether these results show that Six and Eya genes lie genetically upstream of Pax3 gene in the formation of ventrolateral dermomyotome hypaxial lips. No genetic links have been characterized between Six and Eya genes, but corresponding proteins activate key muscle determination genes (Myod, Myogenin and Mrf4). These results establish a new hierarchy of genes controlling early steps of hypaxial myogenic commitment in the mouse embryo.
KW - Eyes absent/Eya proteins
KW - Hypaxial
KW - Muscle
KW - OFC syndrome
KW - Pax3
KW - Six/sine oculis homeoproteins
KW - Somite
UR - http://www.scopus.com/inward/record.url?scp=33846879582&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2006.08.059
DO - 10.1016/j.ydbio.2006.08.059
M3 - Article
C2 - 17098221
AN - SCOPUS:33846879582
SN - 0012-1606
VL - 302
SP - 602
EP - 616
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -