Extracts of protozoa contain materials that react specifically in the immunoassay for guinea pig insulin

F. de Pablo, M. A. Lesniak, E. R. Hernandez, D. LeRoith, J. Shiloach, J. Roth

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Extracts of protozoa contain materials that resemble guinea pig insulin, which is noted for its unusual structure and properties. The protozoan derived materials react in the radioimmunoassay for guinea pig insulin; some but not all of these immunoactive materials migrate on gel filtration in the position of authentic guinea pig insulin. Experiments were done to exclude artifacts in the assay as well as inadvertent contamination by guinea pig insulin. By immunological methods, we segregated the guinea pig type immunoactivity from that which has rat/pork type immunoactivity. These findings extend our studies of extracts of guinea pig tissues which also have these two types of insulin immunoactivities.

Original languageEnglish
Pages (from-to)82-87
Number of pages6
JournalHormone and Metabolic Research
Volume18
Issue number2
DOIs
StatePublished - 1986

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