Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

Jun Kyu Kim; Young Jin Youn; Yu Bin Lee; Sun Hwa Kim; Dong Keun Song; Minsang Shin; Hee Kyung Jin; Jae sung Bae; Sanjeeb Shrestha; Chang Won Hong

doi:10.1038/s41598-021-87891-8

Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

Jun Kyu Kim
, Young Jin Youn
, Yu Bin Lee
, Sun Hwa Kim
, Dong Keun Song
, Minsang Shin
, Hee Kyung Jin
, Jae sung Bae
, Sanjeeb Shrestha
, Chang Won Hong

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.

Original language	English
Article number	8289
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-87891-8
State	Published - Dec 2021
Externally published	Yes

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title = "Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics",

abstract = "Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.",

author = "Kim, \{Jun Kyu\} and Youn, \{Young Jin\} and Lee, \{Yu Bin\} and Kim, \{Sun Hwa\} and Song, \{Dong Keun\} and Minsang Shin and Jin, \{Hee Kyung\} and Bae, \{Jae sung\} and Sanjeeb Shrestha and Hong, \{Chang Won\}",

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doi = "10.1038/s41598-021-87891-8",

language = "English",

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AU - Kim, Jun Kyu

AU - Youn, Young Jin

AU - Lee, Yu Bin

AU - Kim, Sun Hwa

AU - Song, Dong Keun

AU - Shin, Minsang

AU - Jin, Hee Kyung

AU - Bae, Jae sung

AU - Shrestha, Sanjeeb

AU - Hong, Chang Won

PY - 2021/12

Y1 - 2021/12

N2 - Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.

AB - Extracellular vesicles (EVs) are membrane-derived heterogeneous vesicles that mediate intercellular communications. They have recently been considered as ideal vehicles for drug-delivery systems, and immune cells are suggested as a potential source for drug-loaded EVs. In this study, we investigated the possibility of neutrophils as a source for drug-loaded EVs. Neutrophil-like differentiated human promyelocytic leukemia cells (dHL-60) produced massive amounts of EVs within 1 h. The dHL-60 cells are also easily loaded with various cargoes such as antibiotics (penicillin), anticancer drug (paclitaxel), chemoattractant (MCP-1), miRNA, and Cas9. The EVs derived from the dHL-60 cells showed efficient incorporation of these cargoes and significant effector functions, such as bactericidal activity, monocyte chemotaxis, and macrophage polarization. Our results suggest that neutrophils or neutrophil-like promyelocytic cells could be an attractive source for drug-delivery EVs.

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JO - Scientific Reports

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Extracellular vesicles from dHL-60 cells as delivery vehicles for diverse therapeutics

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