Expression, purification, and isotope labeling of the Fv of the human HIV-1 neutralizing antibody 447-52D for NMR studies

Keyphrases

• Human Immunodeficiency Virus Type 1 (HIV-1) 100%
• Escherichia Coli 100%
• NMR Studies 100%
• HIV Antibodies 100%
• Isotopic Labeling 100%
• Structure Determination 100%
• Antibody Gene 100%
• Single-chain Fv 100%
• Bicistronic Vector 100%
• Virus 50%
• Envelope Glycoprotein 50%
• Monoclonal Antibody 50%
• Gp120 50%
• Recombinant 50%
• Expression Level 50%
• Restriction Sites 50%
• Polypeptide 50%
• Infected Patients 50%
• Signal Peptide 50%
• V3 Loop 50%
• Variable Loop 50%
• Periplasm 50%
• Heavy Chain 50%
• Immunodominant 50%
• Neutralizing Epitope 50%
• Light Chain 50%
• Human Antibody 50%
• Oligonucleotides 50%
• Leader Peptide 50%
• NMR Spectra 50%
• Antigen-binding Site 50%
• Third Variables 50%
• Variable Domains 50%
• Multinuclear NMR 50%
• Individual Expression 50%
• Termination Codon 50%
• Constant Region 50%
• Antibody Sequence 50%
• Fv Fragment 50%

Immunology and Microbiology

• Human Immunodeficiency Virus 1 100%
• Neutralizing Antibody 100%
• Isotope Labeling 100%
• Peptides 75%
• Escherichia coli 50%
• Single-Chain Variable Fragment 50%
• Structure Determination 50%
• Human Monoclonal Antibody 25%
• Epitope 25%
• Nuclear Magnetic Resonance Spectroscopy 25%
• Secretion (Process) 25%
• Codon 25%
• Solution and Solubility 25%
• Periplasmic Space 25%
• Heavy Chain 25%
• Light Chain 25%
• Envelope Glycoprotein GP120 25%
• Expression Vector 25%
• Restriction Site 25%
• Virus 25%
• Fragment Antigen-Binding 25%