TY - JOUR
T1 - Expression of three distinct families of calcium-activated chloride channel genes in the mouse dorsal root ganglion
AU - Al-Jumaily, Mohammed
AU - Kozlenkov, Alexei
AU - Mechaly, Ilana
AU - Fichard, Agnes
AU - Matha, Valerie
AU - Scamps, Frederique
AU - Valmier, Jean
AU - Carroll, Patrick
N1 - Funding Information:
Acknowledgements: This work was supported by grants from Association Française contre les Myopathies, a scholarship from the French Ministry of Foreign Affairs, and a fellowship from the French Ministry of Research. We acknowledge the expert technical assistance of Sylvie Maillé.
PY - 2007/9
Y1 - 2007/9
N2 - Objective: A calcium-activated chloride current (ICl(Ca)) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current. Methods: Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have ICl(Ca) current inducing properties. Results: We showed the detection of transcripts representing several members of these families, i.e. chloride channel calciumactivated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion: Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced ICl(Ca) in DRG neurons.
AB - Objective: A calcium-activated chloride current (ICl(Ca)) has been observed in medium-sized sensory neurons of the dorsal root ganglion (DRG). Axotomy of the sciatic nerve induces a similar current in the majority of medium and large diameter neurons. Our aim is to identify the molecule(s) underlying this current. Methods: Using conventional and quantitative RT-PCR, we examined the expression in DRG of members of three families of genes, which have been shown to have ICl(Ca) current inducing properties. Results: We showed the detection of transcripts representing several members of these families, i.e. chloride channel calciumactivated (CLCA), Bestrophin and Tweety gene families in adult DRG, in the normal state and 3 d after sciatic nerve section, a model for peripheral nerve injury. Conclusion: Our analysis revealed that that mBest1 and Tweety2 appear as the best candidates to play a role in the injury-induced ICl(Ca) in DRG neurons.
KW - Bestrophin
KW - Chloride channel
KW - Chloride channel calcium activated
KW - Dorsal root ganglion
KW - Tweety
UR - http://www.scopus.com/inward/record.url?scp=36549009056&partnerID=8YFLogxK
U2 - 10.1007/s12264-007-0044-8
DO - 10.1007/s12264-007-0044-8
M3 - Article
C2 - 17952139
AN - SCOPUS:36549009056
SN - 1673-7067
VL - 23
SP - 293
EP - 299
JO - Neuroscience Bulletin
JF - Neuroscience Bulletin
IS - 5
ER -