Expression of the normal human sis/PDGF-2 coding sequence induces cellular transformation

Arnona Gazit, Hisanaga Igarashi, Ing Ming Chiu, Alagarsamy Srinivasan, Abraham Yaniv, Steven R. Tronick, Keith C. Robbins, Stuart A. Aaronson

Research output: Contribution to journalArticlepeer-review

120 Scopus citations


The human sis proto-oncogene contains the coding sequence for one of two polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH 3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. However, c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kb sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. Thus the normal coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
Issue number1
StatePublished - Nov 1984
Externally publishedYes


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