TY - JOUR
T1 - Expression of the normal human sis/PDGF-2 coding sequence induces cellular transformation
AU - Gazit, Arnona
AU - Igarashi, Hisanaga
AU - Chiu, Ing Ming
AU - Srinivasan, Alagarsamy
AU - Yaniv, Abraham
AU - Tronick, Steven R.
AU - Robbins, Keith C.
AU - Aaronson, Stuart A.
PY - 1984/11
Y1 - 1984/11
N2 - The human sis proto-oncogene contains the coding sequence for one of two polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH 3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. However, c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kb sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. Thus the normal coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.
AB - The human sis proto-oncogene contains the coding sequence for one of two polypeptide chains present in preparations of biologically active human platelet-derived growth factor (PDGF). A human clone, c-sis clone 8, which contains all of the v-sis-related sequences present in human DNA, was transcriptionally inactive when transfected into NIH 3T3 cells. When placed under the control of a retrovirus LTR, the clone was transcribed at levels comparable to that observed in cells transformed by SSV DNA. However, c-sis clone 8 DNA did not express detectable sis/PDGF-2 proteins and lacked biologic activity. A putative upstream exon was identified by its ability to detect the 4.2 kb sis-related transcript in certain human cells. When this sequence was inserted in the proper orientation between the LTR and c-sis clone 8, the chimeric molecule acquired high titered transforming activity, comparable to that of SSV DNA. Transformants containing this construct expressed human sis/PDGF-2 translational products. Thus the normal coding sequence for a human growth factor has transforming activity when expressed in an appropriate assay cell.
UR - http://www.scopus.com/inward/record.url?scp=0021703127&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(84)90194-6
DO - 10.1016/0092-8674(84)90194-6
M3 - Article
C2 - 6091919
AN - SCOPUS:0021703127
SN - 0092-8674
VL - 39
SP - 89
EP - 97
JO - Cell
JF - Cell
IS - 1
ER -