Expression of cellular-yes protein in mammalian tissues

Expression of the c-yes proto-oncogene in normal mammalian tissues was studied by immunohistochemistry and by immunoprecipitation assays using affinity purified anti-yes IgG. The antibody was raised against the aminoterminal domain of the human c-yes protein expressed in bacteria as a trpE-yes fusion protein. We detected p62^c-yes in a variety of rat and human tissues but its relative level of expression varied significantly among different cell types. Certain epithelial cells, platelets and spermatid acrosomes showed the highest levels of p62^c-yes. Intracellular localization of p62^c-yes in epithelial cells differed among organs performing different physiological functions. The overall pattern of expression suggests that p62^c-yes may be involved in numerous cellular pathways.