Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

Luc P. Brion; Wendy Cammer; Lisa M. Satlin; Carlos Suarez; Beth J. Zavilowitz; Victor L. Schuster

doi:10.1152/ajprenal.1997.273.2.f234

Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

Luc P. Brion, Wendy Cammer, Lisa M. Satlin, Carlos Suarez, Beth J. Zavilowitz, Victor L. Schuster

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

Original language	English
Pages (from-to)	F234-F245
Journal	American Journal of Physiology - Renal Physiology
Volume	273
Issue number	2 42-2
DOIs	https://doi.org/10.1152/ajprenal.1997.273.2.f234
State	Published - Aug 1997
Externally published	Yes

Keywords

Affinity chromatography
Carbonic anhydrase II deficiency
Carbonic anhydrase isozymes I, II, III, and IV
Cortex
Hydratase activity
Liver
Lung
Outer medulla
Regulation
Renal tubular acidosis

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10.1152/ajprenal.1997.273.2.f234

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title = "Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice",

abstract = "Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.",

keywords = "Affinity chromatography, Carbonic anhydrase II deficiency, Carbonic anhydrase isozymes I, II, III, and IV, Cortex, Hydratase activity, Liver, Lung, Outer medulla, Regulation, Renal tubular acidosis",

author = "Brion, {Luc P.} and Wendy Cammer and Satlin, {Lisa M.} and Carlos Suarez and Zavilowitz, {Beth J.} and Schuster, {Victor L.}",

year = "1997",

month = aug,

doi = "10.1152/ajprenal.1997.273.2.f234",

language = "English",

volume = "273",

pages = "F234--F245",

journal = "American Journal of Physiology - Renal Physiology",

issn = "1931-857X",

publisher = "American Physiological Society",

number = "2 42-2",

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T1 - Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

AU - Brion, Luc P.

AU - Cammer, Wendy

AU - Satlin, Lisa M.

AU - Suarez, Carlos

AU - Zavilowitz, Beth J.

AU - Schuster, Victor L.

PY - 1997/8

Y1 - 1997/8

N2 - Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

AB - Chronic metabolic acidosis (CMA) in the rabbit upregulates carbonic anhydrase (CA) IV in the proximal convoluted tubule (PCT). This study was designed to assess CA IV expression in a model of CMA in the mouse, i.e., congenital deficiency in CA II [CA(II)D]. In female CA(II)D mice, CA IV specific activity but not CA IV immunoreactivity was upregulated in the renal cortex, specifically in microdissected PCTs. Western blot analysis showed higher expression of CA IV immunoreactive protein in renal membranes from males than in those from females.

KW - Affinity chromatography

KW - Carbonic anhydrase II deficiency

KW - Carbonic anhydrase isozymes I, II, III, and IV

KW - Cortex

KW - Hydratase activity

KW - Liver

KW - Lung

KW - Outer medulla

KW - Regulation

KW - Renal tubular acidosis

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U2 - 10.1152/ajprenal.1997.273.2.f234

DO - 10.1152/ajprenal.1997.273.2.f234

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AN - SCOPUS:0030846260

SN - 1931-857X

VL - 273

SP - F234-F245

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

IS - 2 42-2

ER -

Expression of carbonic anhydrase IV in carbonic anhydrase II-deficient mice

Abstract

Keywords

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