Expression of basic fibroblast growth factor and angiogenin in arthritis

S. Hosaka, M. R. Shah, N. Barquin, G. K. Haines, A. E. Koch

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Angiogenesis is an integral component of the vasculoproliferative phase of rheumatoid arthritis (RA). We examined the distribution of two angiogenic factors, basic fibroblast growth factor (bFGF) and angiogenin (ANG) in arthritic diseases. We used an enzyme-linked immunosorbent assay and immunohistochemical analysis to determine the levels of bFGF and ANG in synovial fluid (SF) and their distribution in synovial tissue (ST), respectively. Most SF contained little or no detectable bFGF (< 5 pg/ml). ANG in RA SF was 248.7 ± 17.4 ng/ml, which did not differ significantly from levels found in osteoarthritis (OA; 305.9 ± 23.1 ng/ml). Synovial lining cells, macrophages, endothelial cells, and vascular smooth muscle cells were immunopositive for bFGF and ANG; however, their expression was not up-regulated in RA ST compared to ST from OA and normal subjects. Though bFGF and ANG are present in the joints of patients with arthritic diseases, they are not up-regulated in RA. These results suggest that not all angiogenic mediators are up-regulated in RA compared to normal subjects and subjects with other arthritic diseases. It may be that some of these mediators, like ANG, play a role in the physiology of normal synovium.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalPathobiology
Volume63
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • Angiogenic factor
  • Angiogenin
  • Basic fibroblast growth factor
  • Rheumatoid arthritis
  • Synovial fluid

Fingerprint

Dive into the research topics of 'Expression of basic fibroblast growth factor and angiogenin in arthritis'. Together they form a unique fingerprint.

Cite this