Abstract
Pw1/Peg3 is a parentally imprinted gene expressed in adult stem cells in every tissue thus far examined including the stem cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the stem cells in the bulge, which is a major stem cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the bulge stem cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34+ and CD34− populations of bulge stem cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle stem cells and reveal a novel CD34− bulge stem-cell population. Stem Cells 2017;35:1015–1027.
Original language | English |
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Pages (from-to) | 1015-1027 |
Number of pages | 13 |
Journal | Stem Cells |
Volume | 35 |
Issue number | 4 |
DOIs | |
State | Published - 1 Apr 2017 |
Externally published | Yes |
Keywords
- Bulge stem cells
- Cell cycle
- Pw1/Peg3