Experimental perfusion system utilizing the rabbit Vx-2 carcinoma II. Correlative studies of in vitro sterilization of Vx-2 cells with results of perfusions in rabbits and in patients

Shyunichi Mori, Bayard Clarkson, Annabel O'Connor, Walter Lawrence

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3 Scopus citations

Abstract

The effect of exposure to six chemotherapeutic agents incubated with Vx-2 carcinoma cells in vitro has been determined by injecting cells into rabbits. 5-Fluoro-2′-deoxyuridine, methotrexate, and cyclophosphamide failed to cause sterilization o f Vx-2 cells in vitro and had little influence on subsequent tumor growth. HN2, actinomycin D, and mitomycin C all produced sterilization of Vx-2 cells during in vitro incubation. The minimal sterilizing concentration of each of these drugs was established. The results o f the in vitro tests were compared with the plasma concentration in the perfusion circuit during experimental rabbit hind limb perfusions as well as during pelvic perfusions in patients using these same drugs when suitable assay procedures were available (5-fluoro-2′-deoxyuridine, methotrexate, actinomycin D, and mitomycin C:). Except for cyclophosphamide, there was generally good correlation between the results of the in vitro tests with Vx-2 cells and the therapeutic effect of perfusions.

Original languageEnglish
Pages (from-to)447-463
Number of pages17
JournalClinical Pharmacology and Therapeutics
Volume3
Issue number4
DOIs
StatePublished - 1962
Externally publishedYes

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