Experimental annotation of the human genome using microarray technology

D. D. Shoemaker, E. E. Schadt, C. D. Armour, Y. D. He, P. Garrett-Engele, P. D. McDonagh, P. M. Loerch, A. Leonardson, P. Y. Lum, G. Cavet, L. F. Wu, S. J. Altschuler, S. Edwards, J. King, J. S. Tsang, G. Schimmack, J. M. Schelter, J. Koch, M. Ziman, M. J. MartonB. Li, P. Cundiff, T. Ward, J. Castle, M. Krolewski, M. R. Meyer, M. Mao, J. Burchard, M. J. Kidd, H. Dai, J. W. Phillips, P. S. Linsley, R. Stoughton, S. Scherer, M. S. Boguski

Research output: Contribution to journalArticlepeer-review

374 Scopus citations

Abstract

The most important product of the sequencing of a genome is a complete, accurate catalogue of genes and their products, primarily messenger RNA transcripts and their cognate proteins. Such a catalogue cannot be constructed by computational annotation alone; it requires experimental validation on a genome scale. Using 'exon' and 'tiring' arrays fabricated by ink-jet oligonucleotide synthesis, we devised an experimental approach to validate and refine computational gene predictions and define full-length transcripts on the basis of co-regulated expression of their exons. These methods can provide more accurate gene numbers and allow the detection of mRNA splice variants and identification of the tissue- and disease-specific conditions under which genes are expressed. We apply our technique to chromosome 22q under 69 experimental condition pairs, and to the entire human genome under two experimental conditions. We discuss implications for more comprehensive, consistent and reliable genome annotation, more efficient, full-length complementary DNA cloning strategies and application to complex diseases.

Original languageEnglish
Pages (from-to)922-927
Number of pages6
JournalNature
Volume409
Issue number6822
DOIs
StatePublished - 15 Feb 2001
Externally publishedYes

Fingerprint

Dive into the research topics of 'Experimental annotation of the human genome using microarray technology'. Together they form a unique fingerprint.

Cite this