Expansion of transplanted hepatocytes during liver regeneration

Nada A. Yazigi, Terri L. Carrick, John C. Bucuvalas, Charles S. Schmidt, William F. Balistreri, Jorge A. Bezerra

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Background. Successful clinical application of hepatocyte transplantation has been limited by poor engraftment of the recipient liver by transplanted hepatocytes. Methods. To address the hypothesis that liver regeneration induced by an acute hepatotoxic injury promotes expansion of transplanted hepatocytes, we injected β-galactosidase-labeled hepatocytes intrasplenically into mice 24 hr after treatment with carbon tetrachloride (CCl4) and into untreated controls. Results. Macroscopic examination of whole liver segments identified clusters of transplanted hepatocytes uniformly spread on the capsular surface of the recipient liver and in the liver core following the distribution pattern of portal vein branches. Frozen sections showed that although the degree of initial engraftment of transplanted hepatocytes was similar in CCl4 treated and control livers, there was a fourfold increase of engrafted hepatocytes in CCl4-treated livers 10 days after transplantation which persisted to 28 days. Conclusions. We conclude that the number of transplanted hepatocytes increases in response to regeneration signal triggered by an acute hepatocyte-specific liver injury.

Original languageEnglish
Pages (from-to)816-820
Number of pages5
Issue number6
StatePublished - 27 Sep 1997
Externally publishedYes


Dive into the research topics of 'Expansion of transplanted hepatocytes during liver regeneration'. Together they form a unique fingerprint.

Cite this