TY - JOUR
T1 - Expanding the psoriasis disease profile
T2 - Interrogation of the skin and serum of patients with moderate-to-severe psoriasis
AU - Suárez-Fariñas, Mayte
AU - Li, Katherine
AU - Fuentes-Duculan, Judilyn
AU - Hayden, Karen
AU - Brodmerkel, Carrie
AU - Krueger, James G.
N1 - Funding Information:
We thank the ACCEPT Biopsy Investigators for collecting the biopsy samples, and Michelle Perate (Janssen) for editorial assistance. Funding for this work was provided by Janssen Research & Development LLC, a Johnson & Johnson pharmaceutical company.
PY - 2012/11
Y1 - 2012/11
N2 - Psoriasis is a complex disease with an expanding definition of its pathological features. We sought to expand/refine the psoriasis transcriptome using 85 paired lesional and non-lesional samples from a cohort of patients with moderate-to-severe psoriasis vulgaris who were not receiving active psoriasis therapy. This new analysis identified 4,175 probe sets (representing 2,725 unique known genes) as being differentially expressed in psoriasis lesions compared with matched biopsies of non-lesional skin when the following criteria were applied: >2-fold change and false discovery rate <0.05. These probe sets represent the largest and most comprehensive set of genes defining psoriasis at the molecular level and within the previously unidentified genes, a link to functional pathways associated with metabolic diseases/diabetes and to cardiovascular risk pathways is identified. In addition, we profiled the serum of moderate-to-severe psoriatics compared with healthy controls to assess the overlap of overexpressed lesional genes with overexpressed systemic proteins. We identified linkage of functional pathways in lesional skin associated with metabolic diseases/diabetes and cardiovascular risk with those pathways overexpressed in the serum, suggesting a potential linkage between altered gene transcription in the skin and comorbidities commonly seen in patients with moderate-to-severe psoriasis.
AB - Psoriasis is a complex disease with an expanding definition of its pathological features. We sought to expand/refine the psoriasis transcriptome using 85 paired lesional and non-lesional samples from a cohort of patients with moderate-to-severe psoriasis vulgaris who were not receiving active psoriasis therapy. This new analysis identified 4,175 probe sets (representing 2,725 unique known genes) as being differentially expressed in psoriasis lesions compared with matched biopsies of non-lesional skin when the following criteria were applied: >2-fold change and false discovery rate <0.05. These probe sets represent the largest and most comprehensive set of genes defining psoriasis at the molecular level and within the previously unidentified genes, a link to functional pathways associated with metabolic diseases/diabetes and to cardiovascular risk pathways is identified. In addition, we profiled the serum of moderate-to-severe psoriatics compared with healthy controls to assess the overlap of overexpressed lesional genes with overexpressed systemic proteins. We identified linkage of functional pathways in lesional skin associated with metabolic diseases/diabetes and cardiovascular risk with those pathways overexpressed in the serum, suggesting a potential linkage between altered gene transcription in the skin and comorbidities commonly seen in patients with moderate-to-severe psoriasis.
UR - http://www.scopus.com/inward/record.url?scp=84867887920&partnerID=8YFLogxK
U2 - 10.1038/jid.2012.184
DO - 10.1038/jid.2012.184
M3 - Article
C2 - 22763790
AN - SCOPUS:84867887920
SN - 0022-202X
VL - 132
SP - 2552
EP - 2564
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 11
ER -