Excluded volume effects on the rate of renaturation of DNA

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Abstract

The rate of renaturation of T2 DNA hits been investigated by using complementary DNA strands of different length. The length of the shorter strand ranged from 0.02 to 1.0 times the length of the longer strand. An excluded volume theory is developed to include this type of reaction as well as the DNA–RNA hybridization reaction. Experimental and theoretical rates of renaturation of DNA are found to be in agreement. For the cases studied, the rate was never greater than twice that observed for short strands of the same length renaturing with themselves. The products of renaturation reactions are also considered.

Original languageEnglish
Pages (from-to)601-613
Number of pages13
JournalBiopolymers
Volume10
Issue number4
DOIs
StatePublished - Apr 1971
Externally publishedYes

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