Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

Patrizia Casaccia-Bonnefil; Eirikur Benedikz; Rabindra Rai; Peter J. Bergold

doi:10.1016/0304-3940(93)90157-G

Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

Patrizia Casaccia-Bonnefil
, Eirikur Benedikz
, Rabindra Rai
, Peter J. Bergold

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-d-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.

Original language	English
Pages (from-to)	5-8
Number of pages	4
Journal	Neuroscience Letters
Volume	154
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90157-G
State	Published - 14 May 1993
Externally published	Yes

Keywords

Hippocampus
Kainate
Mossy fiber
N-Methyl-d-aspartate
Organotypic
Picrotoxin

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10.1016/0304-3940(93)90157-G

Cite this

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title = "Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures",

abstract = "In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-d-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.",

keywords = "Hippocampus, Kainate, Mossy fiber, N-Methyl-d-aspartate, Organotypic, Picrotoxin",

author = "Patrizia Casaccia-Bonnefil and Eirikur Benedikz and Rabindra Rai and Bergold, \{Peter J.\}",

note = "Funding Information: We thank R. Sloviter for assistance with the Timm sulphide staining, M.A.Q. Siddiqui for the generous use of the tissue culture facilities and T. Sacktor and A. Stel-zer for reading this manuscript. This work is supported by funds from SUNY-HSCB and the Epilepsy Foundation of America. P. Casaccia-Bonnefil is a recipient of the SUNY-HSCB Competitive Fellowship.",

year = "1993",

month = may,

day = "14",

doi = "10.1016/0304-3940(93)90157-G",

language = "English",

volume = "154",

pages = "5--8",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

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TY - JOUR

T1 - Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

AU - Casaccia-Bonnefil, Patrizia

AU - Benedikz, Eirikur

AU - Rai, Rabindra

AU - Bergold, Peter J.

N1 - Funding Information: We thank R. Sloviter for assistance with the Timm sulphide staining, M.A.Q. Siddiqui for the generous use of the tissue culture facilities and T. Sacktor and A. Stel-zer for reading this manuscript. This work is supported by funds from SUNY-HSCB and the Epilepsy Foundation of America. P. Casaccia-Bonnefil is a recipient of the SUNY-HSCB Competitive Fellowship.

PY - 1993/5/14

Y1 - 1993/5/14

N2 - In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-d-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.

AB - In organotypic hippocampal slice cultures, kainate (KA) specifically induces cell loss in the CA3 region while N-methyl-d-aspartate induces cell loss in the CA1 region. The sensitivity of slice cultures to KA toxicity appears only after 2 weeks in vitro which parallels the appearance of mossy fibers. KA toxicity is potentiated by co-application with the GABA-A antagonist, picrotoxin. These data suggest that the excitotoxicity of KA in slice cultures is modulated by both excitatory and inhibitory synapses.

KW - Hippocampus

KW - Kainate

KW - Mossy fiber

KW - N-Methyl-d-aspartate

KW - Organotypic

KW - Picrotoxin

UR - https://www.scopus.com/pages/publications/0027288197

U2 - 10.1016/0304-3940(93)90157-G

DO - 10.1016/0304-3940(93)90157-G

M3 - Article

C2 - 8361647

AN - SCOPUS:0027288197

SN - 0304-3940

VL - 154

SP - 5

EP - 8

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Excitatory and inhibitory pathways modulate kainate excitotoxicity in hippocampal slice cultures

Abstract

Keywords

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