Exact cleavage site of Alzheimer amyloid precursor in neuronal PC-12 cells

John P. Anderson; Fred S. Esch; Pamela S. Keim; Kumar Sambamurti; Ivan Lieberburg; Nikolaos K. Robakis

doi:10.1016/0304-3940(91)90775-O

Exact cleavage site of Alzheimer amyloid precursor in neuronal PC-12 cells

John P. Anderson, Fred S. Esch, Pamela S. Keim, Kumar Sambamurti, Ivan Lieberburg, Nikolaos K. Robakis

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Abstract

We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys¹⁶-Leu¹⁷ bond in the β amyloid peptide, followed by partial removal of Lys¹⁶ by a basic carboxypeptidase.

Original language	English
Pages (from-to)	126-128
Number of pages	3
Journal	Neuroscience Letters
Volume	128
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90775-O
State	Published - 8 Jul 1991

Keywords

Alzheimer amyloid precursor protein
Alzheimer disease
PC-12 cell
Secretory protease

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title = "Exact cleavage site of Alzheimer amyloid precursor in neuronal PC-12 cells",

abstract = "We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the β amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.",

keywords = "Alzheimer amyloid precursor protein, Alzheimer disease, PC-12 cell, Secretory protease",

author = "Anderson, {John P.} and Esch, {Fred S.} and Keim, {Pamela S.} and Kumar Sambamurti and Ivan Lieberburg and Robakis, {Nikolaos K.}",

note = "Funding Information: We thank Ms. Maryanne Maniatis for fine technical assistance. This work was supported by NIA Grants AG08200, AG05138 and the McKnight Foundation for Neuroscience.",

year = "1991",

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doi = "10.1016/0304-3940(91)90775-O",

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T1 - Exact cleavage site of Alzheimer amyloid precursor in neuronal PC-12 cells

AU - Anderson, John P.

AU - Esch, Fred S.

AU - Keim, Pamela S.

AU - Sambamurti, Kumar

AU - Lieberburg, Ivan

AU - Robakis, Nikolaos K.

N1 - Funding Information: We thank Ms. Maryanne Maniatis for fine technical assistance. This work was supported by NIA Grants AG08200, AG05138 and the McKnight Foundation for Neuroscience.

PY - 1991/7/8

Y1 - 1991/7/8

N2 - We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the β amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.

AB - We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the β amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.

KW - Alzheimer amyloid precursor protein

KW - Alzheimer disease

KW - PC-12 cell

KW - Secretory protease

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1

ER -

Exact cleavage site of Alzheimer amyloid precursor in neuronal PC-12 cells

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Keywords

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