We have identified the secretory cleavage site in the Alzheimer amyloid precursor (APP) in a non-transfected neuronal cell line, using cyanogen bromide digests of APP purified from medium conditioned by PC-12 cells which were differentiated to a neuronal phenotype. The results obtained are most consistent with proteolysis of the Lys16-Leu17 bond in the β amyloid peptide, followed by partial removal of Lys16 by a basic carboxypeptidase.
- Alzheimer amyloid precursor protein
- Alzheimer disease
- PC-12 cell
- Secretory protease