Abstract
Adult skeletal muscle stem cells, termed satellite cells, are essential for regenerating muscle after tissue damage. Satellite cells are located in a specialized microenvironment between muscle fibers and their surrounding basal lamina. This local niche serves as a compartment to preserve satellite cell function and provides signals that facilitate the rapid response to injury. Visualization of this local niche enables the elucidation of such niche-derived signals. Here, we describe techniques for isolating single myofibers with their associated satellite cells for ex vivo visualization and analysis of an intact muscle stem cell niche.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 39-50 |
| Number of pages | 12 |
| DOIs | |
| State | Published - 2019 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2002 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Keywords
- Cadherin
- Catenin
- Cell adhesion
- Integrin
- Muscle
- Niche
- Quiescence
- Regeneration
- Satellite cell
- Stem cell
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