Ex vivo expansion of adult hematopoietic stem and progenitor cells with valproic acid

Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, Ronald Hoffman

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

Umbilical cord blood (UCB) units provide an alternative source of human hematopoietic stem cells (HSCs) for patients who require allogeneic stem cell transplantation but lack a matched donor. However, the limited number of HSCs within each UCB unit remains a major challenge for their use in regenerative medicine and HSC transplantation in adults. Efficient expansion of human HSCs in ex vivo cultures initiated with CD34+ cells isolated from UCBs can overcome this limitation. The method described here utilizes a deacetylase inhibitor, valproic acid (VPA), to rapidly expand to a high degree the numbers of functional HSCs and committed progenitors (HPCs). The expanded HSCs are capable of establishing both short-term and long-term multilineage hematopoietic reconstitution. This highly reproducible and simple protocol can be also applied to expansion of both HSCs and HPCs from different sources including the bone marrow and peripheral blood.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages267-280
Number of pages14
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2185
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cryopreservation
  • Ex vivo expansion
  • Hematopoietic progenitor cells (HPCs)
  • Hematopoietic stem cells (HSCs)
  • Umbilical cord blood (UCB)
  • Valproic acid (VPA)

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