TY - JOUR
T1 - Ex vivo bioluminescence imaging of late gestation ewes following intrauterine inoculation with lux-modified Escherichia coli
AU - Moulton, K.
AU - Ryan, P.
AU - Christiansen, D.
AU - Hopper, R.
AU - Klauser, C.
AU - Bennett, W.
AU - Rodts-Palenik, S.
AU - Willard, S.
N1 - Funding Information:
This work was supported by the USDA-ARS-funded Biophotonics Initiative #58-6402-3-0120 and is a contribution to the W-173 Multi-State Regional Research Project: Stress Factors and Effects on Performance in Livestock. The authors also gratefully acknowledge the Department of Animal and Dairy Sciences and the Mississippi Agriculture and Forestry Experiment Station for study resource support. The authors thank Dr. Sherrill Fleming and Dr. Dawn Tucker (CVM-MSU) for veterinary assistance.
PY - 2009/9
Y1 - 2009/9
N2 - Our objectives were to develop an ovine model for Escherichia coli-induced preterm delivery, and monitor E. coli (lux modified for photonic detection) invasion of the fetal environment-ewes (124 ± 18 d of gestation) received intrauterine inoculations using E. coli-lux as follows: control (n = 5), 1.2 × 106 CFU/ml (n = 5), 5.6 × 106 CFU/ml (n = 5) E. coli-lux. Preterm delivery occurred between 48 and 120 h post-inoculation in 60%, 60% of ewes infected with 1.2, and 5.6 × 106 CFU/ml E. coli-lux, respectively, with presence of emitting bacteria confirmed by real-time imaging of lamb tissues. In summary, preterm delivery and/or fetal distress were observed in a majority of inoculated ewes. Finally, the use of photonic bacteria with imaging was a feasible means to monitor bacterial presence ex vivo.
AB - Our objectives were to develop an ovine model for Escherichia coli-induced preterm delivery, and monitor E. coli (lux modified for photonic detection) invasion of the fetal environment-ewes (124 ± 18 d of gestation) received intrauterine inoculations using E. coli-lux as follows: control (n = 5), 1.2 × 106 CFU/ml (n = 5), 5.6 × 106 CFU/ml (n = 5) E. coli-lux. Preterm delivery occurred between 48 and 120 h post-inoculation in 60%, 60% of ewes infected with 1.2, and 5.6 × 106 CFU/ml E. coli-lux, respectively, with presence of emitting bacteria confirmed by real-time imaging of lamb tissues. In summary, preterm delivery and/or fetal distress were observed in a majority of inoculated ewes. Finally, the use of photonic bacteria with imaging was a feasible means to monitor bacterial presence ex vivo.
KW - E. coli
KW - Photonic
KW - Pregnant ewes
UR - https://www.scopus.com/pages/publications/63349090819
U2 - 10.1016/j.cimid.2008.02.001
DO - 10.1016/j.cimid.2008.02.001
M3 - Article
C2 - 18440069
AN - SCOPUS:63349090819
SN - 0147-9571
VL - 32
SP - 429
EP - 438
JO - Comparative Immunology, Microbiology and Infectious Diseases
JF - Comparative Immunology, Microbiology and Infectious Diseases
IS - 5
ER -