TY - JOUR
T1 - Evolutionary relatedness of the predicted gene product of RNA segment 2 of the Tick-Borne Dhori virus and the PB1 polymerase gene of influenza viruses
AU - Lin, Debby A.
AU - Roychoudhury, Sonali
AU - Palese, Peter
AU - Clay, William C.
AU - Fuller, Frederick J.
N1 - Funding Information:
This work was supported by National Institutes Al-l 8998 (P-P.), Al-l 1823 (P.P.) and Al-20939 (F.F.). in partial fulfillment of a Westinghouse project (D.L.).
PY - 1991/5
Y1 - 1991/5
N2 - The complete nucleotide sequence of the second largest RNA segment of Dhori/India/1313/61 virus was determined and the deduced amino acid sequence was compared with the polymerase (P) proteins of influenza A, B, and C viruses. RNA segment 2 (2224 nucleotides) of Dhori virus contains a single long open reading frame that can encode a 716-amino amid polypeptide (81.3 kDa). The predicted polypeptide shares between 27 and 31% sequence identities with the PB1 polypeptides of influenza A, B, and C viruses. Among the regions most highly conserved are the sequences around the Asp-Asp motif common to many RNA polymerases. In spite of the high level of sequence identity between the Dhori RNA segment 2 gene product and the influenza A, B, and C virus PB1 proteins the amino acid composition of the Dhori protein indicates an acidic charge feature at pH 7.0 in contrast to the basic nature of the PB1 proteins of the influenza viruses. We suggest that the Dhori PB1-like protein be designated the Pα protein of this virus.
AB - The complete nucleotide sequence of the second largest RNA segment of Dhori/India/1313/61 virus was determined and the deduced amino acid sequence was compared with the polymerase (P) proteins of influenza A, B, and C viruses. RNA segment 2 (2224 nucleotides) of Dhori virus contains a single long open reading frame that can encode a 716-amino amid polypeptide (81.3 kDa). The predicted polypeptide shares between 27 and 31% sequence identities with the PB1 polypeptides of influenza A, B, and C viruses. Among the regions most highly conserved are the sequences around the Asp-Asp motif common to many RNA polymerases. In spite of the high level of sequence identity between the Dhori RNA segment 2 gene product and the influenza A, B, and C virus PB1 proteins the amino acid composition of the Dhori protein indicates an acidic charge feature at pH 7.0 in contrast to the basic nature of the PB1 proteins of the influenza viruses. We suggest that the Dhori PB1-like protein be designated the Pα protein of this virus.
UR - http://www.scopus.com/inward/record.url?scp=0025892222&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(91)90641-N
DO - 10.1016/0042-6822(91)90641-N
M3 - Article
C2 - 2024457
AN - SCOPUS:0025892222
SN - 0042-6822
VL - 182
SP - 1
EP - 7
JO - Virology
JF - Virology
IS - 1
ER -