TY - JOUR
T1 - Evidence that a ryanodine receptor triggers signal transduction in the osteoclast
AU - Zaidi, Mone
AU - Shankar, Vijai S.
AU - Towhidul Alam, A. S.M.
AU - Moonga, Baljit S.
AU - Pazianas, Michael
AU - Huang, Christopher L.H.
N1 - Funding Information:
Acknowledgments: A.S.M.T.A. is recipient of the Overseas Research Scholars Award. This work was supported by the Medical Research Council, U.K. (M.Z.), Arthritis and Rheumatism Council, U.K. (M.Z.) and Leverhulme Trust, U.K. (M.Z.). We are grateful to Mr. W. Smith (Cambridge) for expert technical assistance and to Professors I. MacIntyre and R. H. Adrian for advice and encouragement.
PY - 1992/11/16
Y1 - 1992/11/16
N2 - We have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+. Ni2+ (6 mM) in the presence of ethyleneglycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and valinomycin (5 μM) induced a transient elevation of cytosolic [Ca2+] in fura 2-loaded osteoclasts. This transient was superimposed upon a small steady elevation of cytosolic [Ca2+] induced by the initial application of valinomycin alone. Ryanodine (10 μM) completely abolished such responsiveness. However, cytosolic [Ca2+] transients were restored when osteoclasts were depolarized by the extracellular inclusion of 100 mM-[K+] in the same solution. Thus, we demonstrate a sensitivity of the osteoclast signal transduction system to ryanodine for the first time to our knowledge.
AB - We have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+. Ni2+ (6 mM) in the presence of ethyleneglycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and valinomycin (5 μM) induced a transient elevation of cytosolic [Ca2+] in fura 2-loaded osteoclasts. This transient was superimposed upon a small steady elevation of cytosolic [Ca2+] induced by the initial application of valinomycin alone. Ryanodine (10 μM) completely abolished such responsiveness. However, cytosolic [Ca2+] transients were restored when osteoclasts were depolarized by the extracellular inclusion of 100 mM-[K+] in the same solution. Thus, we demonstrate a sensitivity of the osteoclast signal transduction system to ryanodine for the first time to our knowledge.
UR - https://www.scopus.com/pages/publications/0026473070
U2 - 10.1016/0006-291X(92)91377-3
DO - 10.1016/0006-291X(92)91377-3
M3 - Article
C2 - 1445365
AN - SCOPUS:0026473070
SN - 0006-291X
VL - 188
SP - 1332
EP - 1336
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -