Evidence that a ryanodine receptor triggers signal transduction in the osteoclast

Mone Zaidi, Vijai S. Shankar, A. S.M. Towhidul Alam, Baljit S. Moonga, Michael Pazianas, Christopher L.H. Huang

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

We have investigated the effect of the alkaloid ryanodine on the release of intracellularly stored Ca2+ in response to activation of the osteoclast Ca2+ receptor by the surrogate agonist, Ni2+. Ni2+ (6 mM) in the presence of ethyleneglycol bis-(aminoethyl ether) tetraacetic acid (EGTA) (1.2 mM) and valinomycin (5 μM) induced a transient elevation of cytosolic [Ca2+] in fura 2-loaded osteoclasts. This transient was superimposed upon a small steady elevation of cytosolic [Ca2+] induced by the initial application of valinomycin alone. Ryanodine (10 μM) completely abolished such responsiveness. However, cytosolic [Ca2+] transients were restored when osteoclasts were depolarized by the extracellular inclusion of 100 mM-[K+] in the same solution. Thus, we demonstrate a sensitivity of the osteoclast signal transduction system to ryanodine for the first time to our knowledge.

Original languageEnglish
Pages (from-to)1332-1336
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume188
Issue number3
DOIs
StatePublished - 16 Nov 1992
Externally publishedYes

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