TY - JOUR
T1 - Evidence for RNA linked to nascent DNA in hela cells
AU - Des Gouttes Olgiati, Dominique
AU - Pogo, Beatriz G.T.
AU - Dales, Samuel
PY - 1976/3/1
Y1 - 1976/3/1
N2 - Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double-stranded conformation. Moreover, analysis by nitrocellulose chromatography and CsSO4 density sedimentation of the nascent DNA labeled with 3H-uridine revealed that it is covalently linked to short chains of RNA, indicating that in HeLa cells RNA primer is involved in DNA replication.
AB - Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double-stranded conformation. Moreover, analysis by nitrocellulose chromatography and CsSO4 density sedimentation of the nascent DNA labeled with 3H-uridine revealed that it is covalently linked to short chains of RNA, indicating that in HeLa cells RNA primer is involved in DNA replication.
UR - http://www.scopus.com/inward/record.url?scp=0017257713&partnerID=8YFLogxK
U2 - 10.1083/jcb.68.3.557
DO - 10.1083/jcb.68.3.557
M3 - Article
C2 - 1030704
AN - SCOPUS:0017257713
SN - 0021-9525
VL - 68
SP - 557
EP - 566
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -