Evaluation of Mn exposure in the male reproductive system and its relationship with reproductive dysfunction in mice

Tugstênio L. Souza, Amândia R. Batschauer, Patricia Manuitt Brito, Joelma Leão-Buchir, Katherinne Maria Spercoski, Francisco Filipak Neto, Anderson J. Martino-Andrade, Claudia F. Ortolani-Machado

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Manganese (Mn) is essential for animal development and homeostasis. However, anthropogenic activities increase the concentration of Mn in the environment and lead to increased risk of exposure to high doses of the metal. Thus, this study aimed to evaluate the effect of high doses of Mn on the male reproductive system of swiss mice. The 22-day old mice were randomly sorted into four groups and exposed to 0 (control), 15, 30 and 60 mg of MnCl2/kg/day, via daily gavages for 45 days. After the exposure, the mice were euthanized and sperm, hormonal and oxidative stress endpoints were evaluated in the testis, seminal vesicle and hypothalamus. Exposure to Mn promoted weight reduction of androgen-dependent organs, as well as alteration of the levels of fecal androgenic metabolites. Sperm parameters were drastically affected in all treated groups and the antioxidants tested (catalase and glutathione-disulfide reductase activities, and non-protein thiols content) decreased in the testis. However, only a few endpoints were altered in the seminal vesicle. For the hypothalamus, there was a reduction in acetylcholinesterase activity, suggesting a neurotoxic potential of Mn. In conclusion, Mn may affect the hypothalamic-gonadal axis by impairing the development of androgen-dependent organs, testicular redox status and Leydig cell maturation.

Original languageEnglish
Article number152504
StatePublished - Aug 2020
Externally publishedYes


  • Leydig cell
  • Metal toxicity
  • Oxidative stress
  • Sperm analysis


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