TY - JOUR
T1 - Evaluation of counterions for electrospray ionization mass spectral analysis of a highly sulfated carbohydrate, sucrose octasulfate
AU - Gunay, Nur Sibel
AU - Tadano-Aritomi, Keiko
AU - Toida, Toshihiko
AU - Ishizuka, Ineo
AU - Linhardt, Robert J.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other sulfated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1°, 2°, and 3° ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode.
AB - A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other sulfated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1°, 2°, and 3° ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode.
UR - http://www.scopus.com/inward/record.url?scp=0037999940&partnerID=8YFLogxK
U2 - 10.1021/ac034053l
DO - 10.1021/ac034053l
M3 - Article
C2 - 12964773
AN - SCOPUS:0037999940
SN - 0003-2700
VL - 75
SP - 3226
EP - 3231
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 13
ER -