Evaluation of counterions for electrospray ionization mass spectral analysis of a highly sulfated carbohydrate, sucrose octasulfate

Nur Sibel Gunay, Keiko Tadano-Aritomi, Toshihiko Toida, Ineo Ishizuka, Robert J. Linhardt

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A systematic approach was used to evaluate the electrospray ionization mass spectral (ESI-MS) analysis of sucrose octasulfate (SOS), an important pharmaceutical agent. SOS represents a model for other sulfated carbohydrates, such as heparin and glycosaminoglycan-derived oligosaccharides that also are highly sulfated and pose difficult analytical problems. A survey of ammonium counterions showed that 1°, 2°, and 3° ammonium salts of SOS gave substantial fragmentation as a result of sulfate loss. In contrast, quaternary ammonium and phosphonium salts gave excellent ESI spectra, particularly in the positive ion mode. This represents the first report of the ESI-MS analysis of sulfated carbohydrates in the positive ion mode.

Original languageEnglish
Pages (from-to)3226-3231
Number of pages6
JournalAnalytical Chemistry
Volume75
Issue number13
DOIs
StatePublished - 1 Jul 2003
Externally publishedYes

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