TY - JOUR
T1 - Evaluation of anti-osteoporotic activity of butanolic fraction from Passiflora foetida in ovariectomy-induced bone loss in mice
AU - Ahmad, Naseer
AU - Chillara, Raju
AU - Kushwaha, Priyanka
AU - Khedgikar, Vikram
AU - Karvande, Anirudha
AU - Choudhary, Dharmendra
AU - Adhikary, Sulekha
AU - Maurya, Rakesh
AU - Trivedi, Ritu
N1 - Publisher Copyright:
© 2017 Elsevier Masson SAS
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Objective In this study, we have evaluated the skeletal effects of butanolic fraction (BF) from Passiflora foetida in an estrogen deficient mice bone loss model. Study design Skeletal effect of BF was studied in ovariectomized (OVx) female Balb/c mice. BF (50 and 100 mg/kg/day dose orally) was given for 8 weeks. Micro-architecture of long bones, biomechanical strength, formations of mineralized nodule by bone marrow osteoprogenitor cells, osteoid formation and bone turnover markers were studied. One way ANOVA was used to test the significance of effects of Passiflora foetida. Results OVx mice treated with BF represented with better micro-architectural parameters at various anatomical positions, better bone biomechanical strength and more osteoprogenitor cells in the bone marrow compared with OVx group. BF did not exhibit uterine estrogenicity. Conclusion Oral administration of BF at both the doses (50 and 100 mg/kg/day) derived from Passiflora Foetida, was found to afford anti-osteoporotic effect under estrogen deficiency by likely stimulation of osteoblast function and inhibition of osteoclast function.
AB - Objective In this study, we have evaluated the skeletal effects of butanolic fraction (BF) from Passiflora foetida in an estrogen deficient mice bone loss model. Study design Skeletal effect of BF was studied in ovariectomized (OVx) female Balb/c mice. BF (50 and 100 mg/kg/day dose orally) was given for 8 weeks. Micro-architecture of long bones, biomechanical strength, formations of mineralized nodule by bone marrow osteoprogenitor cells, osteoid formation and bone turnover markers were studied. One way ANOVA was used to test the significance of effects of Passiflora foetida. Results OVx mice treated with BF represented with better micro-architectural parameters at various anatomical positions, better bone biomechanical strength and more osteoprogenitor cells in the bone marrow compared with OVx group. BF did not exhibit uterine estrogenicity. Conclusion Oral administration of BF at both the doses (50 and 100 mg/kg/day) derived from Passiflora Foetida, was found to afford anti-osteoporotic effect under estrogen deficiency by likely stimulation of osteoblast function and inhibition of osteoclast function.
KW - Estrogenicity
KW - Microcomputed tomography
KW - Osteogenic
KW - Ovariectomy
KW - Trabecular microarchitecture
UR - http://www.scopus.com/inward/record.url?scp=85011332648&partnerID=8YFLogxK
U2 - 10.1016/j.biopha.2017.01.100
DO - 10.1016/j.biopha.2017.01.100
M3 - Article
C2 - 28160755
AN - SCOPUS:85011332648
SN - 0753-3322
VL - 88
SP - 804
EP - 813
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
ER -