Estrogen stimulation of galanin gene expression and galanin-like immunoreactivity in the rat and its blockade by the estrogen antagonist keoxifene (LY156758)

Steven M. Gabriel; James I. Koenig; Debra L. Washton

doi:10.1016/0167-0115(93)90367-H

Estrogen stimulation of galanin gene expression and galanin-like immunoreactivity in the rat and its blockade by the estrogen antagonist keoxifene (LY156758)

Steven M. Gabriel, James I. Koenig, Debra L. Washton

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Rat galanin (rGAL) gene expression is stimulated potently by 17β-estradiol in the anterior pituitary. Neuroendocrine tissue extracts of were purified by chromatography and analyzed for rGAL-like (-LI) immunoreactivity. Greater than 90% of rGAL-LI eluted at the same position as the synthetic rGAL standard in untreated anterior pituitary, median eminence and neurointermediate lobe tissues. Additional immunoreactive forms were detected in the hypothalamus, anterior pituitary and MtT/W15 adenoma tissues, particularly after 17β-estradiol treatment. We examined rGAL and its encoding mRNA in the anterior pituitary of immature female rats after the injection of pregnant mare serum gonadotropin (PMSG). One and two days after PMSG injection, serum 17β-estradiol increased 3-fold and 4-fold, respectively. This resulted in a surge of endogenous gonadotropin 2 days after PMSG. At this time, rGAL-encoding mRNA was increased 40-fold over controls. Three days after PMSG, there was a 6-fold increase in anterior pituitary and a 41% increase in plasma rGAL-LI concentrations. Plasma 17β-estradiol one day after injection of PMSG and the consequent anterior pituitary rGAL-LI concentrations 2 days later were positively correlated. This stimulation of rGAL and its encoding mRNA by PMSG was inhibited by treatment with the estrogen antagonist keoxifene (LY156758).

Original language	English
Pages (from-to)	407-419
Number of pages	13
Journal	Regulatory Peptides
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/0167-0115(93)90367-H
State	Published - 11 Jun 1993

Keywords

Anterior pituitary
Antiestrogen
Chromatography
Luteinizing hormone
Pregnant mare serum gonadotropin
Puberty

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10.1016/0167-0115(93)90367-H

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@article{4049c63f92334cfabaa51ad65c648ac7,

title = "Estrogen stimulation of galanin gene expression and galanin-like immunoreactivity in the rat and its blockade by the estrogen antagonist keoxifene (LY156758)",

abstract = "Rat galanin (rGAL) gene expression is stimulated potently by 17β-estradiol in the anterior pituitary. Neuroendocrine tissue extracts of were purified by chromatography and analyzed for rGAL-like (-LI) immunoreactivity. Greater than 90% of rGAL-LI eluted at the same position as the synthetic rGAL standard in untreated anterior pituitary, median eminence and neurointermediate lobe tissues. Additional immunoreactive forms were detected in the hypothalamus, anterior pituitary and MtT/W15 adenoma tissues, particularly after 17β-estradiol treatment. We examined rGAL and its encoding mRNA in the anterior pituitary of immature female rats after the injection of pregnant mare serum gonadotropin (PMSG). One and two days after PMSG injection, serum 17β-estradiol increased 3-fold and 4-fold, respectively. This resulted in a surge of endogenous gonadotropin 2 days after PMSG. At this time, rGAL-encoding mRNA was increased 40-fold over controls. Three days after PMSG, there was a 6-fold increase in anterior pituitary and a 41% increase in plasma rGAL-LI concentrations. Plasma 17β-estradiol one day after injection of PMSG and the consequent anterior pituitary rGAL-LI concentrations 2 days later were positively correlated. This stimulation of rGAL and its encoding mRNA by PMSG was inhibited by treatment with the estrogen antagonist keoxifene (LY156758).",

keywords = "Anterior pituitary, Antiestrogen, Chromatography, Luteinizing hormone, Pregnant mare serum gonadotropin, Puberty",

author = "Gabriel, {Steven M.} and Koenig, {James I.} and Washton, {Debra L.}",

note = "Funding Information: The authors thank Dr. Lee Kaplan for donating the rGAL-2 plasmid and consultations on this manuscript, Dr. Margaret Niedenthal at Lilly Research Laboratories for the generous gift of keoxifene used in this study and Dr. Salvatore Raiti for the rLH reagents. We would also like to acknowledge the excellent technical assistance of Ms. Nancy Ruiz and Mr. Jaime Roncancio. This work was supported by grant MH45480 (SMG) and presented at the 1991 Neuroscience Meeting, New Orleans, LA.",

year = "1993",

month = jun,

day = "11",

doi = "10.1016/0167-0115(93)90367-H",

language = "English",

volume = "45",

pages = "407--419",

journal = "Regulatory Peptides",

issn = "0167-0115",

publisher = "Elsevier",

number = "3",

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T1 - Estrogen stimulation of galanin gene expression and galanin-like immunoreactivity in the rat and its blockade by the estrogen antagonist keoxifene (LY156758)

AU - Gabriel, Steven M.

AU - Koenig, James I.

AU - Washton, Debra L.

N1 - Funding Information: The authors thank Dr. Lee Kaplan for donating the rGAL-2 plasmid and consultations on this manuscript, Dr. Margaret Niedenthal at Lilly Research Laboratories for the generous gift of keoxifene used in this study and Dr. Salvatore Raiti for the rLH reagents. We would also like to acknowledge the excellent technical assistance of Ms. Nancy Ruiz and Mr. Jaime Roncancio. This work was supported by grant MH45480 (SMG) and presented at the 1991 Neuroscience Meeting, New Orleans, LA.

PY - 1993/6/11

Y1 - 1993/6/11

N2 - Rat galanin (rGAL) gene expression is stimulated potently by 17β-estradiol in the anterior pituitary. Neuroendocrine tissue extracts of were purified by chromatography and analyzed for rGAL-like (-LI) immunoreactivity. Greater than 90% of rGAL-LI eluted at the same position as the synthetic rGAL standard in untreated anterior pituitary, median eminence and neurointermediate lobe tissues. Additional immunoreactive forms were detected in the hypothalamus, anterior pituitary and MtT/W15 adenoma tissues, particularly after 17β-estradiol treatment. We examined rGAL and its encoding mRNA in the anterior pituitary of immature female rats after the injection of pregnant mare serum gonadotropin (PMSG). One and two days after PMSG injection, serum 17β-estradiol increased 3-fold and 4-fold, respectively. This resulted in a surge of endogenous gonadotropin 2 days after PMSG. At this time, rGAL-encoding mRNA was increased 40-fold over controls. Three days after PMSG, there was a 6-fold increase in anterior pituitary and a 41% increase in plasma rGAL-LI concentrations. Plasma 17β-estradiol one day after injection of PMSG and the consequent anterior pituitary rGAL-LI concentrations 2 days later were positively correlated. This stimulation of rGAL and its encoding mRNA by PMSG was inhibited by treatment with the estrogen antagonist keoxifene (LY156758).

AB - Rat galanin (rGAL) gene expression is stimulated potently by 17β-estradiol in the anterior pituitary. Neuroendocrine tissue extracts of were purified by chromatography and analyzed for rGAL-like (-LI) immunoreactivity. Greater than 90% of rGAL-LI eluted at the same position as the synthetic rGAL standard in untreated anterior pituitary, median eminence and neurointermediate lobe tissues. Additional immunoreactive forms were detected in the hypothalamus, anterior pituitary and MtT/W15 adenoma tissues, particularly after 17β-estradiol treatment. We examined rGAL and its encoding mRNA in the anterior pituitary of immature female rats after the injection of pregnant mare serum gonadotropin (PMSG). One and two days after PMSG injection, serum 17β-estradiol increased 3-fold and 4-fold, respectively. This resulted in a surge of endogenous gonadotropin 2 days after PMSG. At this time, rGAL-encoding mRNA was increased 40-fold over controls. Three days after PMSG, there was a 6-fold increase in anterior pituitary and a 41% increase in plasma rGAL-LI concentrations. Plasma 17β-estradiol one day after injection of PMSG and the consequent anterior pituitary rGAL-LI concentrations 2 days later were positively correlated. This stimulation of rGAL and its encoding mRNA by PMSG was inhibited by treatment with the estrogen antagonist keoxifene (LY156758).

KW - Anterior pituitary

KW - Antiestrogen

KW - Chromatography

KW - Luteinizing hormone

KW - Pregnant mare serum gonadotropin

KW - Puberty

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U2 - 10.1016/0167-0115(93)90367-H

DO - 10.1016/0167-0115(93)90367-H

M3 - Article

C2 - 7688905

AN - SCOPUS:0027247479

SN - 0167-0115

VL - 45

SP - 407

EP - 419

JO - Regulatory Peptides

JF - Regulatory Peptides

IS - 3

ER -

Estrogen stimulation of galanin gene expression and galanin-like immunoreactivity in the rat and its blockade by the estrogen antagonist keoxifene (LY156758)

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Keywords

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