Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

M. Steinitz; I. Seppala; K. Eichmann; G. Klein

doi:10.1016/s0340-904x(79)80060-3

Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

M. Steinitz, I. Seppala, K. Eichmann, G. Klein

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Abstract

A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T₄-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.

Original language	English
Pages (from-to)	41-47
Number of pages	7
Journal	Immunobiology
Volume	156
Issue number	1-2
DOIs	https://doi.org/10.1016/s0340-904x(79)80060-3
State	Published - 1979
Externally published	Yes

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title = "Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate",

abstract = "A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.",

author = "M. Steinitz and I. Seppala and K. Eichmann and G. Klein",

note = "Funding Information: This research was supported (in part) by Contract N01-CP-33316 from the Division of Cancer Cause and Prevention, National Cancer Institute. Support was also received from the Swedish Cancer Society.",

year = "1979",

doi = "10.1016/s0340-904x(79)80060-3",

language = "English",

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journal = "Immunobiology",

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T1 - Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

AU - Steinitz, M.

AU - Seppala, I.

AU - Eichmann, K.

AU - Klein, G.

N1 - Funding Information: This research was supported (in part) by Contract N01-CP-33316 from the Division of Cancer Cause and Prevention, National Cancer Institute. Support was also received from the Swedish Cancer Society.

PY - 1979

Y1 - 1979

N2 - A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.

AB - A human lymphoblastoid cell line, secreting specific antibody against Group A carbohydrate (A-CHO) was established by pre-selection of antigen binding normal human lymphocytes, followed by Epstein-Barr virus (EBV) induced immortalization. Culture supernatants were assayed for anti A-CHO antibodies by radioimmunoassay, N-acetyl-glucosamine-coupled T4-phage plaque inhibition tests and passive hemagglutination. As a rule, the supernatants contained about 10 μg/ml anti-A-CHO antibodies of the IgM-kappa type. The antibody was fractionated and partially purified on an N-acetyl glucosamine Sepharose 4B column with a recovery of about 3 μg/ml of supernatant.

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DO - 10.1016/s0340-904x(79)80060-3

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Establishment of a human lymphoblastoid cell line with specific antibody production against group A streptococcal carbohydrate

Abstract

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