Abstract
The control of IL-2 gene expression in T cells by multiple transcriptional factors has been extensively explored, however, the role of the NF-κB signaling pathway in TCR-dependent IL-2 production still remains unclear. In this study, we used a somatic cell genetics approach to address this question. Triggering TCR in mutant Jurkat T cells lacking IKKγ/NEMO failed to induce IL-2 due to a selective loss in I-κB kinase activity, I-κBα degradation and NF-κB DNA-binding activity. The AP-1 and NF-AT binding activities in the IL-2 promoter were comparable between wild-type and mutant T cells. These defects in the mutant cell line were rescued by the reintroduction of exogenous IKKγ. Taken together, our data demonstrate that IKKγ plays an essential role in TCR-induced signaling pathways leading to IL-2 expression.
Original language | English |
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Pages (from-to) | 1917-1924 |
Number of pages | 8 |
Journal | European Journal of Immunology |
Volume | 33 |
Issue number | 7 |
DOIs | |
State | Published - 1 Jul 2003 |
Keywords
- IKKγ
- IL-2
- NEMO
- NF-κB
- Signaling
- TCR