TY - JOUR
T1 - Erythropoietic differentiation in humans
T2 - In vitro studies on erythroid progenitors and Hb synthesis in fetal, newborn and adult life
AU - Peschle, C.
AU - Migliaccio, G.
AU - Migliaccio, A. R.
AU - Lettieri, F.
AU - Russo, G.
AU - Gianni, A. M.
AU - Ottolenghi, S.
AU - Comi, P.
AU - Giglioni, B.
PY - 1980
Y1 - 1980
N2 - Human erythroid progenitors from fetal liver, cord or adult blood and adult marrow were cultured in methylcellulose, according to standard techniques. Their clonogenetic features (colony morphology and number, time/growth curve, erythropoietin (Ep) and burst enhancing factor (BEF) sensitivity, in vitro 3H-thymidine suicide index, etc) were comparatively investigated. Three classes of fetal liver erythroid progenitors (primitive or intermediate BFU-E, CFU-E) have been thereby identified and characterized. Furthermore, globin chains (α, β, Gγ, Aγ) synthesis has been evaluated in single erythroid colonies, either well or poorly hemoglobinized, by means of a novel technique including analytical iso-electric focusing (IEF), sometimes preceded by preparative IEF separation of HbF and HbA. On the basis of these results, a model for the regulation of Hb synthesis is proposed.
AB - Human erythroid progenitors from fetal liver, cord or adult blood and adult marrow were cultured in methylcellulose, according to standard techniques. Their clonogenetic features (colony morphology and number, time/growth curve, erythropoietin (Ep) and burst enhancing factor (BEF) sensitivity, in vitro 3H-thymidine suicide index, etc) were comparatively investigated. Three classes of fetal liver erythroid progenitors (primitive or intermediate BFU-E, CFU-E) have been thereby identified and characterized. Furthermore, globin chains (α, β, Gγ, Aγ) synthesis has been evaluated in single erythroid colonies, either well or poorly hemoglobinized, by means of a novel technique including analytical iso-electric focusing (IEF), sometimes preceded by preparative IEF separation of HbF and HbA. On the basis of these results, a model for the regulation of Hb synthesis is proposed.
UR - http://www.scopus.com/inward/record.url?scp=0019288261&partnerID=8YFLogxK
M3 - Article
C2 - 7349637
AN - SCOPUS:0019288261
SN - 0301-472X
VL - 8
SP - 153
EP - 159
JO - Experimental Hematology
JF - Experimental Hematology
IS - Suppl. 8
ER -