Erratum: Insulin stimulates PKC -mediated phosphorylation of insulin receptor substrate-1 (IRS-1): A self-attenuated mechanism to negatively regulate the function of IRS proteins (Journal of Biological Chemistry (2001) 276 (14459–14465) DOI: 10.1074/jbc.M007281200)

This article has been withdrawn by Sanford R. Sampson, Motoi Ohba, Toshio Kuroki, Derek LeRoith, and Yehiel Zick. The withdrawing authors have become aware of several errors in the way images were presented in this manuscript. Because the original data are no longer available, the authors wish to withdraw the article in the interests of maintaining their publication standards and those of the journal. In the IRS-1 immunoblot in Fig. 3, lanes 1, 2, and 8 were duplicated. The PKC immunoblot from Fig. 4B was reused in Fig. 5. In Fig. 5, lanes 9 and 12 of the IRS-1 (IR-IRS-1 complex) immunoblot were duplicated. Additionally, lanes 1 and 8 and lanes 5, 10, and 11 of the IRS-1 (total) immunoblot were duplicated. In Fig. 6, lanes 2 and 9 of the IRS-1 immunoblot were duplicated. The withdrawing authors state that these presentational errors do not impact the underlying scientific findings of the article, which are also presented as quantitative bar graphs that essentially summarize data of a number of experiments. Therefore, the withdrawing authors stand by the original scientific results as described, which in the authors’ opinion has been confirmed in other laboratories (Moeschel, K., Beck, A., Weigert, C., Lammers, R., Kalbacher, H., Voelter, W., Schleicher, E. D., Häring, H.-U., and Lehmann, R. (2004) Protein kinase C--induced phosphorylation of Ser³¹⁸ in insulin receptor substrate-1 (IRS-1) attenuates the interaction with the insulin receptor and the tyrosine phosphorylation of IRS-1. J. Biol. Chem. 279, 25157–25163 and Neid, M., Datta, K., Stephan, S., Khanna, I., Pal, S., Shaw, L., White, M., and Mukhopadhyay, D. (2004) Role of insulin receptor substrates and protein kinase C- in vascular permeability factor/ vascular endothelial growth factor expression in pancreatic cancer cells. J. Biol. Chem. 279, 3941–3948).