ERcalcistorin/Protein Disulfide Isomerase (PDI): Sequence determination and expression of a cDNA clone encoding a calcium storage protein with PDI activity from endoplasmic reticulum of the sea urchin egg

Hector A. Lucero, Djamel Lebeche, Benjamin Kaminer

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Abstract

Following the purification of a 58-kDa calsequestrin-like protein from the endoplasmic reticulum (ER) of sea urchin eggs (Oberdorf, J. A., Lebeche, D., Head, J. F., and Kaminer, B. (1988) J. Biol. Chem. 263, 6806-6809) and its characterization as a high capacity, low affinity calcium-binding protein (Lebeche, D., and Kaminer, B. (1992) Biochem. J. 287, 741-747), we isolated and sequenced a cDNA encoding for this protein. The deduced 496 amino acids contain a 17-residue NH2-terminal signal peptide, a KDEL COOH-terminal ER retention signal and two thioredoxin-like active site domains, -CGHC-, identical with those in protein disulfide isomerase (PDI). The sea urchin egg protein shares a 55% sequence identity with mammalian PDI and its PDI activity is 30% of the activity of rabbit liver PDI. The corresponding mRNA was found in oocytes, mature eggs, embryos, and differentiated tissues of the sea urchin in varying amounts. COS-7 cells transfected with the cDNA, expressed a 58-kDa protein immunoreactive to antibodies against the sea urchin egg protein. This molecule appears to have a dual function of calcium storage and PDI activity within the ER. We hence redesignate it ERcalcistorin/PDI (ECaSt/ PDI), a protein that is distinct from calsequestrin and calreticulin.

Original languageEnglish
Pages (from-to)23112-23119
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number37
StatePublished - 16 Sep 1994
Externally publishedYes

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