Epstein-Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen

Elena Kashuba; Vladimir Kashuba; Katja Pokrovskaja; George Klein; Laszlo Szekely

doi:10.1038/sj.onc.1203501

Epstein-Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen

Elena Kashuba, Vladimir Kashuba, Katja Pokrovskaja, George Klein, Laszlo Szekely

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

EBNA-3 (also called EBNA-3A) is one of the EBV encoded nuclear antigens that are necessary for B-cell transformation. EBNA-3 is known to target RBPs, nuclear proteins that also interacts with EBNA-2, EBNA-4 and EBNA-6. In order to identify additional EBNA-3 targets, an EBV-transformed human lymphocyte cDNA library was screened in the yeast two-hybrid system with N-terminus truncated EBNA-3 that cannot interact with RBP-Jκ. A clone, encoding Xap-2 protein, a cellular partner of Hepatitis B virus X-antigen was isolated. This protein is also known as the p38 subunit of the aryl hydrocarbon receptor complex (ARA9). The specific binding to EBNA-3 was confirmed by showing that the CST-Xap-2 precipitated EBNA-3 from CV1 cells that were infected with recombinant vaccinia virus expressing EBNA-3. Deletion of the C-terminus of Xap-2 eliminated the binding. Fusion with green fluorescent protein showed that Xap-2 is preferentially cytoplasmic but translocates to the nucleus upon expression of EBNA-3.

Original language	English
Pages (from-to)	1801-1806
Number of pages	6
Journal	Oncogene
Volume	19
Issue number	14
DOIs	https://doi.org/10.1038/sj.onc.1203501
State	Published - 30 Mar 2000
Externally published	Yes

Keywords

EBNA-3
HBV
Xap-2

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@article{a97742972654439ab17e765d9468e4f5,

title = "Epstein-Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen",

abstract = "EBNA-3 (also called EBNA-3A) is one of the EBV encoded nuclear antigens that are necessary for B-cell transformation. EBNA-3 is known to target RBPs, nuclear proteins that also interacts with EBNA-2, EBNA-4 and EBNA-6. In order to identify additional EBNA-3 targets, an EBV-transformed human lymphocyte cDNA library was screened in the yeast two-hybrid system with N-terminus truncated EBNA-3 that cannot interact with RBP-Jκ. A clone, encoding Xap-2 protein, a cellular partner of Hepatitis B virus X-antigen was isolated. This protein is also known as the p38 subunit of the aryl hydrocarbon receptor complex (ARA9). The specific binding to EBNA-3 was confirmed by showing that the CST-Xap-2 precipitated EBNA-3 from CV1 cells that were infected with recombinant vaccinia virus expressing EBNA-3. Deletion of the C-terminus of Xap-2 eliminated the binding. Fusion with green fluorescent protein showed that Xap-2 is preferentially cytoplasmic but translocates to the nucleus upon expression of EBNA-3.",

keywords = "EBNA-3, HBV, Xap-2",

author = "Elena Kashuba and Vladimir Kashuba and Katja Pokrovskaja and George Klein and Laszlo Szekely",

note = "Funding Information: We thank Lena Norrenius for the excellent technical assistance and Klas G Wiman for the GST-ARF plasmids. This work was supported by Cancerfonden and by a matching grant from the Concern Foundation, Los Angeles, the Cancer Research Institute, New York.",

year = "2000",

month = mar,

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doi = "10.1038/sj.onc.1203501",

language = "English",

volume = "19",

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journal = "Oncogene",

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TY - JOUR

T1 - Epstein-Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen

AU - Kashuba, Elena

AU - Kashuba, Vladimir

AU - Pokrovskaja, Katja

AU - Klein, George

AU - Szekely, Laszlo

N1 - Funding Information: We thank Lena Norrenius for the excellent technical assistance and Klas G Wiman for the GST-ARF plasmids. This work was supported by Cancerfonden and by a matching grant from the Concern Foundation, Los Angeles, the Cancer Research Institute, New York.

PY - 2000/3/30

Y1 - 2000/3/30

N2 - EBNA-3 (also called EBNA-3A) is one of the EBV encoded nuclear antigens that are necessary for B-cell transformation. EBNA-3 is known to target RBPs, nuclear proteins that also interacts with EBNA-2, EBNA-4 and EBNA-6. In order to identify additional EBNA-3 targets, an EBV-transformed human lymphocyte cDNA library was screened in the yeast two-hybrid system with N-terminus truncated EBNA-3 that cannot interact with RBP-Jκ. A clone, encoding Xap-2 protein, a cellular partner of Hepatitis B virus X-antigen was isolated. This protein is also known as the p38 subunit of the aryl hydrocarbon receptor complex (ARA9). The specific binding to EBNA-3 was confirmed by showing that the CST-Xap-2 precipitated EBNA-3 from CV1 cells that were infected with recombinant vaccinia virus expressing EBNA-3. Deletion of the C-terminus of Xap-2 eliminated the binding. Fusion with green fluorescent protein showed that Xap-2 is preferentially cytoplasmic but translocates to the nucleus upon expression of EBNA-3.

AB - EBNA-3 (also called EBNA-3A) is one of the EBV encoded nuclear antigens that are necessary for B-cell transformation. EBNA-3 is known to target RBPs, nuclear proteins that also interacts with EBNA-2, EBNA-4 and EBNA-6. In order to identify additional EBNA-3 targets, an EBV-transformed human lymphocyte cDNA library was screened in the yeast two-hybrid system with N-terminus truncated EBNA-3 that cannot interact with RBP-Jκ. A clone, encoding Xap-2 protein, a cellular partner of Hepatitis B virus X-antigen was isolated. This protein is also known as the p38 subunit of the aryl hydrocarbon receptor complex (ARA9). The specific binding to EBNA-3 was confirmed by showing that the CST-Xap-2 precipitated EBNA-3 from CV1 cells that were infected with recombinant vaccinia virus expressing EBNA-3. Deletion of the C-terminus of Xap-2 eliminated the binding. Fusion with green fluorescent protein showed that Xap-2 is preferentially cytoplasmic but translocates to the nucleus upon expression of EBNA-3.

KW - EBNA-3

KW - HBV

KW - Xap-2

UR - https://www.scopus.com/pages/publications/0034732259

U2 - 10.1038/sj.onc.1203501

DO - 10.1038/sj.onc.1203501

M3 - Article

C2 - 10777214

AN - SCOPUS:0034732259

SN - 0950-9232

VL - 19

SP - 1801

EP - 1806

JO - Oncogene

JF - Oncogene

IS - 14

ER -

Epstein-Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen

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