TY - JOUR
T1 - Epstein-Barr virus (EBV) antigen-specific leukocyte migration inhibition (LMI) in infectious mononucleosis (IM). I. Kinetics and response to a membrane protein on EBV-transformed cells
AU - Szigeti, Robert
AU - Timar, Laszlo
AU - Weiland, Ola
AU - Henle, Werner
AU - Henle, Gertrude
AU - Hennessy, Kevin
AU - Kieff, Elliott
AU - Sulitzeanu, Dov
AU - Dillner, Joakim
AU - Klein, George
N1 - Funding Information:
This study was supported by the Swedish Cancer Society and by PHS Grant 2 R01 CA 30264-04 awarded by the National Cancer Institute DHHS (R.S.. J.D.. and G.K.), by Grant R.D. 221 (G.H. and W.H.) and Grant ACS MV 325 from the American Cancer Society and the Cancer Research Foundation of Chicago (K.H. and E.K.). and by a grant from the Concern Foundation, Los Angeles (D.S.). R.S. is a recipient of a research fellowship from the Swedish Cancer Society,
PY - 1986/12
Y1 - 1986/12
N2 - Cell-mediated immune response of mononucleosis (IM) patients to Epstein-Barr virus (EBV)-determined antigens was measured by the leukocyte migration inhibition (LMI) assay. Patients in the acute phase of the disease failed to respond to partially purified nuclear antigen, EBNA, or to cell extracts that contained EBNA as the predominant EBV antigen. They showed a strong specific response to cell extracts enriched in early antigen (EA) and virus capsid antigen (VCA). The LMI response to EBNA appeared in convalescence in parallel with EBNA-specific antibodies, slightly later in children than in adults. Membrane fractions of EBV-carrying, virus nonproducer Raji cells induced an EBV-specific LMI at approximately the same time. A bacterial fusion protein containing the hydrophilic part of the virus-coded membrane antigen of latently EBV-infected cells also induced an EBV-specific response that parallelled the LMI reaction elicited by the Raji membrane fraction. This is in line with our previous finding (D. Sulitzeanu et al., J. Virol. 58, 230, 1986) that this fusion protein shares an epitope with Raji cell membranes.
AB - Cell-mediated immune response of mononucleosis (IM) patients to Epstein-Barr virus (EBV)-determined antigens was measured by the leukocyte migration inhibition (LMI) assay. Patients in the acute phase of the disease failed to respond to partially purified nuclear antigen, EBNA, or to cell extracts that contained EBNA as the predominant EBV antigen. They showed a strong specific response to cell extracts enriched in early antigen (EA) and virus capsid antigen (VCA). The LMI response to EBNA appeared in convalescence in parallel with EBNA-specific antibodies, slightly later in children than in adults. Membrane fractions of EBV-carrying, virus nonproducer Raji cells induced an EBV-specific LMI at approximately the same time. A bacterial fusion protein containing the hydrophilic part of the virus-coded membrane antigen of latently EBV-infected cells also induced an EBV-specific response that parallelled the LMI reaction elicited by the Raji membrane fraction. This is in line with our previous finding (D. Sulitzeanu et al., J. Virol. 58, 230, 1986) that this fusion protein shares an epitope with Raji cell membranes.
UR - https://www.scopus.com/pages/publications/0022979769
U2 - 10.1016/0090-1229(86)90005-X
DO - 10.1016/0090-1229(86)90005-X
M3 - Article
C2 - 3022972
AN - SCOPUS:0022979769
SN - 0090-1229
VL - 41
SP - 342
EP - 350
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 3
ER -