Epstein-Barr virus co-reconstituted with sendai virus envelopes infects Epstein-Barr virus-receptor negative cells

Ilya M. Shapiro; George Klein; David J. Volsky

doi:10.1016/0304-4165(81)90004-0

Epstein-Barr virus co-reconstituted with sendai virus envelopes infects Epstein-Barr virus-receptor negative cells

Ilya M. Shapiro, George Klein, David J. Volsky

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Epstein-Barr virus (EBV) was co-reconstituted with Sendai virus envelopes. The reconstituted 'hybrid' virus could bind and penetrate into EBV-receptor negative cells. Using this approach, T-cell-derived human and mouse leukemia cells, human T-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of EBV-determined antigens and stimulation of DNA synthesis. The T-cell-derived human leukemia line Molt-4, that can absorb EBV but without virus penetration, could be also infected by the reconstituted EBV.

Original language	English
Pages (from-to)	19-24
Number of pages	6
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	676
Issue number	1
DOIs	https://doi.org/10.1016/0304-4165(81)90004-0
State	Published - 5 Aug 1981
Externally published	Yes

Keywords

Epstein-Barr virus
Reconstitution
Sendai virus envelope
Virus receptor

Access to Document

10.1016/0304-4165(81)90004-0

Cite this

@article{0d0cee2120d7466687fc5e0517b8803f,

title = "Epstein-Barr virus co-reconstituted with sendai virus envelopes infects Epstein-Barr virus-receptor negative cells",

abstract = "Epstein-Barr virus (EBV) was co-reconstituted with Sendai virus envelopes. The reconstituted 'hybrid' virus could bind and penetrate into EBV-receptor negative cells. Using this approach, T-cell-derived human and mouse leukemia cells, human T-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of EBV-determined antigens and stimulation of DNA synthesis. The T-cell-derived human leukemia line Molt-4, that can absorb EBV but without virus penetration, could be also infected by the reconstituted EBV.",

keywords = "Epstein-Barr virus, Reconstitution, Sendai virus envelope, Virus receptor",

author = "Shapiro, {Ilya M.} and George Klein and Volsky, {David J.}",

note = "Funding Information: This work was supported by Grant Number 3 R01 CA 14054-0631, awarded by the National Cancer Institute, DHEW and Swedish Cancer Society. D.J.V. is a recipient of a long-term fellowship from the European Molecular Biology Organization. The authors are grateful to Mrs. Barbro Ehlin-Henriksson for her excellent technical assistance.",

year = "1981",

month = aug,

day = "5",

doi = "10.1016/0304-4165(81)90004-0",

language = "English",

volume = "676",

pages = "19--24",

journal = "Biochimica et Biophysica Acta - General Subjects",

issn = "0304-4165",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Epstein-Barr virus co-reconstituted with sendai virus envelopes infects Epstein-Barr virus-receptor negative cells

AU - Shapiro, Ilya M.

AU - Klein, George

AU - Volsky, David J.

N1 - Funding Information: This work was supported by Grant Number 3 R01 CA 14054-0631, awarded by the National Cancer Institute, DHEW and Swedish Cancer Society. D.J.V. is a recipient of a long-term fellowship from the European Molecular Biology Organization. The authors are grateful to Mrs. Barbro Ehlin-Henriksson for her excellent technical assistance.

PY - 1981/8/5

Y1 - 1981/8/5

N2 - Epstein-Barr virus (EBV) was co-reconstituted with Sendai virus envelopes. The reconstituted 'hybrid' virus could bind and penetrate into EBV-receptor negative cells. Using this approach, T-cell-derived human and mouse leukemia cells, human T-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of EBV-determined antigens and stimulation of DNA synthesis. The T-cell-derived human leukemia line Molt-4, that can absorb EBV but without virus penetration, could be also infected by the reconstituted EBV.

AB - Epstein-Barr virus (EBV) was co-reconstituted with Sendai virus envelopes. The reconstituted 'hybrid' virus could bind and penetrate into EBV-receptor negative cells. Using this approach, T-cell-derived human and mouse leukemia cells, human T-lymphocytes and mouse spleen cells were successfully infected as judged by the induction of EBV-determined antigens and stimulation of DNA synthesis. The T-cell-derived human leukemia line Molt-4, that can absorb EBV but without virus penetration, could be also infected by the reconstituted EBV.

KW - Epstein-Barr virus

KW - Reconstitution

KW - Sendai virus envelope

KW - Virus receptor

UR - http://www.scopus.com/inward/record.url?scp=0019796592&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(81)90004-0

DO - 10.1016/0304-4165(81)90004-0

M3 - Article

C2 - 6266500

AN - SCOPUS:0019796592

SN - 0304-4165

VL - 676

SP - 19

EP - 24

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

IS - 1

ER -

Epstein-Barr virus co-reconstituted with sendai virus envelopes infects Epstein-Barr virus-receptor negative cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this