Epithelial inflammation response induced by Shigella flexneri depends on mucin gene expression

Sophie Nutten; Philippe Sansonetti; Guillemette Huet; Caroline Bourdon-Bisiaux; Bertrand Meresse; Jean Frédéric Colombel; Pierre Desreumaux

doi:10.1016/S1286-4579(02)01636-2

Epithelial inflammation response induced by Shigella flexneri depends on mucin gene expression

Sophie Nutten
, Philippe Sansonetti
, Guillemette Huet
, Caroline Bourdon-Bisiaux
, Bertrand Meresse
, Jean Frédéric Colombel
, Pierre Desreumaux

Research output: Contribution to journal › Review article › peer-review

34 Scopus citations

Abstract

The protective effects of different mucin gene profiles on gut protection were assessed by the evaluation of TNFα production by intestinal epithelial cells infected by Shigella flexneri. Three HT-29 cell lines were used: HT29-G^- (enterocyte-like cells, secreting no mucins), HT29-FU (highly expressing MUC2 and MUC4) and HT29-MTX (highly expressing MUC3 and MUC5AC). These cells were infected either by an invasive (M90T) or the control isogenic (BS176) strains of S. flexneri, and TNFα mRNA production was quantified by competitive PCR. In the HT29-G^- cells, M90T induced an increased production of TNFα mRNA compared to BS176, giving a TNFα ratio of 5.6 ± 3.3. In contrast, similar levels of TNFα mRNA were detected in HT29-FU and HT29-MTX cells stimulated with either M90T or BS176, giving ratios of 1.4 ± 1.3 and 1.0 ± 0.1, respectively. The results suggest that mucin genes have abilities to protect epithelial cells against S. flexneri. Furthermore, the difference in the TNFα ratio between the HT29-FU and HT29-MTX cells suggests distinct protective effects for these two mucin-secreting epithelial cells.

Original language	English
Pages (from-to)	1121-1124
Number of pages	4
Journal	Microbes and Infection
Volume	4
Issue number	11
DOIs	https://doi.org/10.1016/S1286-4579(02)01636-2
State	Published - Sep 2002
Externally published	Yes

Keywords

Intestinal epithelial cells
Mucins
Shigella

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10.1016/S1286-4579(02)01636-2

Cite this

@article{1bd0ba418f98477eaef3bff3a5a8ef65,

title = "Epithelial inflammation response induced by Shigella flexneri depends on mucin gene expression",

abstract = "The protective effects of different mucin gene profiles on gut protection were assessed by the evaluation of TNFα production by intestinal epithelial cells infected by Shigella flexneri. Three HT-29 cell lines were used: HT29-G- (enterocyte-like cells, secreting no mucins), HT29-FU (highly expressing MUC2 and MUC4) and HT29-MTX (highly expressing MUC3 and MUC5AC). These cells were infected either by an invasive (M90T) or the control isogenic (BS176) strains of S. flexneri, and TNFα mRNA production was quantified by competitive PCR. In the HT29-G- cells, M90T induced an increased production of TNFα mRNA compared to BS176, giving a TNFα ratio of 5.6 ± 3.3. In contrast, similar levels of TNFα mRNA were detected in HT29-FU and HT29-MTX cells stimulated with either M90T or BS176, giving ratios of 1.4 ± 1.3 and 1.0 ± 0.1, respectively. The results suggest that mucin genes have abilities to protect epithelial cells against S. flexneri. Furthermore, the difference in the TNFα ratio between the HT29-FU and HT29-MTX cells suggests distinct protective effects for these two mucin-secreting epithelial cells.",

keywords = "Intestinal epithelial cells, Mucins, Shigella",

author = "Sophie Nutten and Philippe Sansonetti and Guillemette Huet and Caroline Bourdon-Bisiaux and Bertrand Meresse and Colombel, \{Jean Fr{\'e}d{\'e}ric\} and Pierre Desreumaux",

note = "Funding Information: This study was supported by grants from the Centre Hospitalier et Universitaire de Lille (No. 96/38/9713). pQB3 and pMus-3 are kind gifts from Dr. D. Shire (Sanofi Elf Bio Recherches, France). We thank Annie Luczak and Odile Moreau for technical assistance.",

year = "2002",

month = sep,

doi = "10.1016/S1286-4579(02)01636-2",

language = "English",

volume = "4",

pages = "1121--1124",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson s.r.l.",

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TY - JOUR

T1 - Epithelial inflammation response induced by Shigella flexneri depends on mucin gene expression

AU - Nutten, Sophie

AU - Sansonetti, Philippe

AU - Huet, Guillemette

AU - Bourdon-Bisiaux, Caroline

AU - Meresse, Bertrand

AU - Colombel, Jean Frédéric

AU - Desreumaux, Pierre

N1 - Funding Information: This study was supported by grants from the Centre Hospitalier et Universitaire de Lille (No. 96/38/9713). pQB3 and pMus-3 are kind gifts from Dr. D. Shire (Sanofi Elf Bio Recherches, France). We thank Annie Luczak and Odile Moreau for technical assistance.

PY - 2002/9

Y1 - 2002/9

N2 - The protective effects of different mucin gene profiles on gut protection were assessed by the evaluation of TNFα production by intestinal epithelial cells infected by Shigella flexneri. Three HT-29 cell lines were used: HT29-G- (enterocyte-like cells, secreting no mucins), HT29-FU (highly expressing MUC2 and MUC4) and HT29-MTX (highly expressing MUC3 and MUC5AC). These cells were infected either by an invasive (M90T) or the control isogenic (BS176) strains of S. flexneri, and TNFα mRNA production was quantified by competitive PCR. In the HT29-G- cells, M90T induced an increased production of TNFα mRNA compared to BS176, giving a TNFα ratio of 5.6 ± 3.3. In contrast, similar levels of TNFα mRNA were detected in HT29-FU and HT29-MTX cells stimulated with either M90T or BS176, giving ratios of 1.4 ± 1.3 and 1.0 ± 0.1, respectively. The results suggest that mucin genes have abilities to protect epithelial cells against S. flexneri. Furthermore, the difference in the TNFα ratio between the HT29-FU and HT29-MTX cells suggests distinct protective effects for these two mucin-secreting epithelial cells.

AB - The protective effects of different mucin gene profiles on gut protection were assessed by the evaluation of TNFα production by intestinal epithelial cells infected by Shigella flexneri. Three HT-29 cell lines were used: HT29-G- (enterocyte-like cells, secreting no mucins), HT29-FU (highly expressing MUC2 and MUC4) and HT29-MTX (highly expressing MUC3 and MUC5AC). These cells were infected either by an invasive (M90T) or the control isogenic (BS176) strains of S. flexneri, and TNFα mRNA production was quantified by competitive PCR. In the HT29-G- cells, M90T induced an increased production of TNFα mRNA compared to BS176, giving a TNFα ratio of 5.6 ± 3.3. In contrast, similar levels of TNFα mRNA were detected in HT29-FU and HT29-MTX cells stimulated with either M90T or BS176, giving ratios of 1.4 ± 1.3 and 1.0 ± 0.1, respectively. The results suggest that mucin genes have abilities to protect epithelial cells against S. flexneri. Furthermore, the difference in the TNFα ratio between the HT29-FU and HT29-MTX cells suggests distinct protective effects for these two mucin-secreting epithelial cells.

KW - Intestinal epithelial cells

KW - Mucins

KW - Shigella

UR - https://www.scopus.com/pages/publications/0036753731

U2 - 10.1016/S1286-4579(02)01636-2

DO - 10.1016/S1286-4579(02)01636-2

M3 - Review article

C2 - 12361911

AN - SCOPUS:0036753731

SN - 1286-4579

VL - 4

SP - 1121

EP - 1124

JO - Microbes and Infection

JF - Microbes and Infection

IS - 11

ER -

Epithelial inflammation response induced by Shigella flexneri depends on mucin gene expression

Abstract

Keywords

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