Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide

Nathan C. Boles; Marie Fernandes; Tomasz Swigut; Rajini Srinivasan; Lauren Schiff; Alvaro Rada-Iglesias; Qingjie Wang; Janmeet S. Saini; Thomas Kiehl; Jeffrey H. Stern; Joanna Wysocka; Timothy A. Blenkinsop; Sally Temple

doi:10.1016/j.stemcr.2020.03.009

Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide

Nathan C. Boles, Marie Fernandes, Tomasz Swigut, Rajini Srinivasan, Lauren Schiff, Alvaro Rada-Iglesias, Qingjie Wang, Janmeet S. Saini, Thomas Kiehl, Jeffrey H. Stern, Joanna Wysocka, Timothy A. Blenkinsop, Sally Temple

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

In this article, Temple, Blenkinsop, and colleagues characterize retinal pigment epithelium (RPE) EMT both transcriptionally and epigenetically in a model of PVR and found a gene signature that predicted the inhibitory effect of nicotinamide on both RPE contractility and EMT.

Original language	English
Pages (from-to)	631-647
Number of pages	17
Journal	Stem Cell Reports
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/j.stemcr.2020.03.009
State	Published - 14 Apr 2020

Keywords

contractility
epigenetics
epithelial to mesenchymal transition
mesenchymal-to-epithelial transition
nicotinamide
proliferative vitreoretinopathy
retinal pigment epithelium
whole transcriptome

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10.1016/j.stemcr.2020.03.009

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Boles, N. C., Fernandes, M., Swigut, T., Srinivasan, R., Schiff, L., Rada-Iglesias, A., Wang, Q., Saini, J. S., Kiehl, T., Stern, J. H., Wysocka, J., Blenkinsop, T. A., & Temple, S. (2020). Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide. Stem Cell Reports, 14(4), 631-647. https://doi.org/10.1016/j.stemcr.2020.03.009

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title = "Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide",

abstract = "In this article, Temple, Blenkinsop, and colleagues characterize retinal pigment epithelium (RPE) EMT both transcriptionally and epigenetically in a model of PVR and found a gene signature that predicted the inhibitory effect of nicotinamide on both RPE contractility and EMT.",

keywords = "contractility, epigenetics, epithelial to mesenchymal transition, mesenchymal-to-epithelial transition, nicotinamide, proliferative vitreoretinopathy, retinal pigment epithelium, whole transcriptome",

author = "Boles, {Nathan C.} and Marie Fernandes and Tomasz Swigut and Rajini Srinivasan and Lauren Schiff and Alvaro Rada-Iglesias and Qingjie Wang and Saini, {Janmeet S.} and Thomas Kiehl and Stern, {Jeffrey H.} and Joanna Wysocka and Blenkinsop, {Timothy A.} and Sally Temple",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

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doi = "10.1016/j.stemcr.2020.03.009",

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Boles, NC, Fernandes, M, Swigut, T, Srinivasan, R, Schiff, L, Rada-Iglesias, A, Wang, Q, Saini, JS, Kiehl, T, Stern, JH, Wysocka, J, Blenkinsop, TA & Temple, S 2020, 'Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide', Stem Cell Reports, vol. 14, no. 4, pp. 631-647. https://doi.org/10.1016/j.stemcr.2020.03.009

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T1 - Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide

AU - Boles, Nathan C.

AU - Fernandes, Marie

AU - Swigut, Tomasz

AU - Srinivasan, Rajini

AU - Schiff, Lauren

AU - Rada-Iglesias, Alvaro

AU - Wang, Qingjie

AU - Saini, Janmeet S.

AU - Kiehl, Thomas

AU - Stern, Jeffrey H.

AU - Wysocka, Joanna

AU - Blenkinsop, Timothy A.

AU - Temple, Sally

PY - 2020/4/14

Y1 - 2020/4/14

N2 - In this article, Temple, Blenkinsop, and colleagues characterize retinal pigment epithelium (RPE) EMT both transcriptionally and epigenetically in a model of PVR and found a gene signature that predicted the inhibitory effect of nicotinamide on both RPE contractility and EMT.

AB - In this article, Temple, Blenkinsop, and colleagues characterize retinal pigment epithelium (RPE) EMT both transcriptionally and epigenetically in a model of PVR and found a gene signature that predicted the inhibitory effect of nicotinamide on both RPE contractility and EMT.

KW - contractility

KW - epigenetics

KW - epithelial to mesenchymal transition

KW - mesenchymal-to-epithelial transition

KW - nicotinamide

KW - proliferative vitreoretinopathy

KW - retinal pigment epithelium

KW - whole transcriptome

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SN - 2213-6711

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JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 4

ER -

Epigenomic and Transcriptomic Changes During Human RPE EMT in a Stem Cell Model of Epiretinal Membrane Pathogenesis and Prevention by Nicotinamide

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Keywords

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