TY - JOUR
T1 - Epigenetic profile in chronic lymphocytic leukemia using methylation-specific multiplex ligation-dependent probe amplification
AU - Cosialls, Ana M.
AU - Santidrián, Antonio F.
AU - Coll-Mulet, Llorenç
AU - Iglesias-Serret, Daniel
AU - González-Gironès, Diana M.
AU - Pérez-Perarnau, Alba
AU - Rubio-Patiño, Camila
AU - González-Barca, Eva
AU - Alonso, Esther
AU - Pons, Gabriel
AU - Gil, Joan
PY - 2012/10
Y1 - 2012/10
N2 - Aim: To analyze the methylation status of 35 tumor suppressor genes using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) in chronic lymphocytic leukemia (CLL). Materials & methods: The DNA of 37 samples from patients with CLL, six healthy donors, and Jurkat and Ramos cell lines was analyzed by MS-MLPA. Results: Our results confirm that hypermethylation is a common and not randomly distributed event in CLL, and some genes, such as WT1, CDH13, IGSF4/TSLC1, GATA5, DAPK1 and RARB, are hypermethylated in more than 25% of the analyzed samples. Importantly, MS-MLPA also detected hypermethylation of some genes not reported previously in CLL, and their methylation status was confirmed by bisulfite sequencing. Conclusion: These results indicate that MS-MLPA is a useful technique for the detection of methylation in CLL samples. Selecting CLL-specific methylation targets in order to generate a CLL-specific MS-MLPA probe set could enhance its usefulness as a tool in studies of risk stratification and guiding the best therapeutic decision.
AB - Aim: To analyze the methylation status of 35 tumor suppressor genes using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) in chronic lymphocytic leukemia (CLL). Materials & methods: The DNA of 37 samples from patients with CLL, six healthy donors, and Jurkat and Ramos cell lines was analyzed by MS-MLPA. Results: Our results confirm that hypermethylation is a common and not randomly distributed event in CLL, and some genes, such as WT1, CDH13, IGSF4/TSLC1, GATA5, DAPK1 and RARB, are hypermethylated in more than 25% of the analyzed samples. Importantly, MS-MLPA also detected hypermethylation of some genes not reported previously in CLL, and their methylation status was confirmed by bisulfite sequencing. Conclusion: These results indicate that MS-MLPA is a useful technique for the detection of methylation in CLL samples. Selecting CLL-specific methylation targets in order to generate a CLL-specific MS-MLPA probe set could enhance its usefulness as a tool in studies of risk stratification and guiding the best therapeutic decision.
KW - CLL
KW - DNA methylation
KW - MS-MLPA
KW - epigenetic profiling
UR - https://www.scopus.com/pages/publications/84868560560
U2 - 10.2217/epi.12.40
DO - 10.2217/epi.12.40
M3 - Article
C2 - 23130831
AN - SCOPUS:84868560560
SN - 1750-1911
VL - 4
SP - 491
EP - 501
JO - Epigenomics
JF - Epigenomics
IS - 5
ER -