Enzyme-linked immunospot (Elispot) for single-cell analysis

Sylvia Janetzki, Rachel Rabin

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations


The ELISpot, a heterogeneous immunoassay, is widely used for detection of low abundant analytes. It is a reliable and robust assay to monitor responses of the immune system at the single-cell level by capturing secreted molecules of interest with specific, membrane-bound antibodies. Those molecules are then made visible by a cascade of ELISA-related development steps. The final results are distinct spots on the membrane as an imprint of the cell secreting the captured molecules, not only allowing their quantification but also providing insight on the kinetics and strength of secretion. This chapter describes the optimized protocol steps of the ELISpot technique, important improvements and tools available for the community, and the current expansion of the technique into polyfunctional cell analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages20
StatePublished - 2015

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • B cell assays
  • ELISpot
  • FluoroSpot
  • Immune monitoring
  • T cell assays


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