TY - JOUR
T1 - Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies
AU - Sternås, Lars
AU - Luka, Janos
AU - Kallin, Bengt
AU - Rosén, Anders
AU - Henle, Werner
AU - Henle, Gertrud
AU - Klein, George
PY - 1983
Y1 - 1983
N2 - Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA - cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.
AB - Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA - cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.
KW - Barr virus (EBV)
KW - EBV antigens
KW - ELISA for EBV-induced antigens
KW - ELISA for anti-EBV antibodies
KW - Epstein
KW - anti-EBV antibodies
UR - http://www.scopus.com/inward/record.url?scp=0020571990&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(83)90422-2
DO - 10.1016/0022-1759(83)90422-2
M3 - Article
C2 - 6311908
AN - SCOPUS:0020571990
VL - 63
SP - 171
EP - 185
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 2
ER -