Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies

Lars Sternås, Janos Luka, Bengt Kallin, Anders Rosén, Werner Henle, Gertrud Henle, George Klein

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA - cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.

Original languageEnglish
Pages (from-to)171-185
Number of pages15
JournalJournal of Immunological Methods
Volume63
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • Barr virus (EBV)
  • EBV antigens
  • ELISA for EBV-induced antigens
  • ELISA for anti-EBV antibodies
  • Epstein
  • anti-EBV antibodies

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