Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies

Lars Sternås; Janos Luka; Bengt Kallin; Anders Rosén; Werner Henle; Gertrud Henle; George Klein

doi:10.1016/0022-1759(83)90422-2

Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies

Lars Sternås, Janos Luka, Bengt Kallin, Anders Rosén, Werner Henle, Gertrud Henle, George Klein

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Abstract

Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA - cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.

Original language	English
Pages (from-to)	171-185
Number of pages	15
Journal	Journal of Immunological Methods
Volume	63
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(83)90422-2
State	Published - 1983
Externally published	Yes

Keywords

Barr virus (EBV)
EBV antigens
ELISA for EBV-induced antigens
ELISA for anti-EBV antibodies
Epstein
anti-EBV antibodies

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10.1016/0022-1759(83)90422-2

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title = "Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies",

abstract = "Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA - cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.",

keywords = "Barr virus (EBV), EBV antigens, ELISA for EBV-induced antigens, ELISA for anti-EBV antibodies, Epstein, anti-EBV antibodies",

author = "Lars Stern{\aa}s and Janos Luka and Bengt Kallin and Anders Ros{\'e}n and Werner Henle and Gertrud Henle and George Klein",

year = "1983",

doi = "10.1016/0022-1759(83)90422-2",

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AU - Sternås, Lars

AU - Luka, Janos

AU - Kallin, Bengt

AU - Rosén, Anders

AU - Henle, Werner

AU - Henle, Gertrud

AU - Klein, George

PY - 1983

Y1 - 1983

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KW - ELISA for anti-EBV antibodies

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Enzyme-linked immunosorbent assay for the detection of Epstein-Barr Virus-Induced Antigens and antibodies

Abstract

Keywords

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