Abstract
Two Epstein-Barr virus (EBV)-specific ELISA tests were developed. One, based on the use of crude extracts from virus producer cells highly induced in the presence of Ara C (providing EA + VCA - cells) or in the absence of the drug (providing EA + VCA + cells) is suitable for the detection of antibodies directed against antigen complexes associated with the lytic virus cycle; i.e., EA, VCA and presumably also MA. The second, performed with purified EBNA, can be used for the detection of antibodies to the transformation-associated nuclear antigen. The tests are expected to find application in the dissection of antibody responses of patients to various antigenic subcomponents, the monitoring of EBV-coded antigens during biochemical purification, and the screening of spent media from hybridoma cultures for EBV-specific antibodies.
Original language | English |
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Pages (from-to) | 171-185 |
Number of pages | 15 |
Journal | Journal of Immunological Methods |
Volume | 63 |
Issue number | 2 |
DOIs | |
State | Published - 1983 |
Externally published | Yes |
Keywords
- Barr virus (EBV)
- EBV antigens
- ELISA for EBV-induced antigens
- ELISA for anti-EBV antibodies
- Epstein
- anti-EBV antibodies