Abstract
Morning salivary 17-hydroxyprogesterone (17-OHP) determination is a useful method to diagnose nonclassical 21-hydroxylase deficiency (NC 21-OHD). In ethnic groups with high frequency of NC 21-OHD, mass screening is thought to be necessary. A pilot screening test for NC 21-OHD was performed by measuring morning salivary 17-OHP by enzyme-linked immunosorbent assay (ELISA). Two individuals were found NC 21-OHD from 230 subjects. Results of this ELISA were well correlated with those of established radioimmunoassay. ELISA can be performed in any laboratory because of non-radioactivity and the procedure is very easy by the use of a microplate. This ELISA is suitable for large scale population studies. We conclude that morning salivary 17-OHP determination by ELISA is a useful method for NC 21-OHD screening test.
| Original language | English |
|---|---|
| Pages (from-to) | 43-45 |
| Number of pages | 3 |
| Journal | Hormone and Metabolic Research |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1994 |
| Externally published | Yes |
Keywords
- enzyme-linked immunosorbent assay (ELISA)
- non-classical 21-hydroxylase deficiency (NC 21-OHD)
- salivary 17-hydroxyprogesterone (17-OHP)