TY - JOUR
T1 - Enzyme activation for nonaqueous media
AU - Lee, Moo Yeal
AU - Dordick, Jonathan S.
N1 - Funding Information:
The authors thank Shanghui Hu for helpful contributions and the Biotechnology Research and Development Corporation, the National Science Foundation, and the Dow Chemical Corporation for funding.
PY - 2002/8/1
Y1 - 2002/8/1
N2 - Highly active enzyme formulations can be prepared for use in nonaqueous media. Considerable progress has been made in the past two years on gaining an improved mechanistic understanding of enzyme function and activation in dehydrated environments. This increased fundamental understanding has led to the development of a broad array of techniques for generating active, stable, and enantioselective and regioselective tailored enzymes for synthetically relevant transformations. This, in turn, is resulting in an exponential increase in the opportunities for enzymatic processes to be developed on a commercial scale.
AB - Highly active enzyme formulations can be prepared for use in nonaqueous media. Considerable progress has been made in the past two years on gaining an improved mechanistic understanding of enzyme function and activation in dehydrated environments. This increased fundamental understanding has led to the development of a broad array of techniques for generating active, stable, and enantioselective and regioselective tailored enzymes for synthetically relevant transformations. This, in turn, is resulting in an exponential increase in the opportunities for enzymatic processes to be developed on a commercial scale.
UR - http://www.scopus.com/inward/record.url?scp=0036669618&partnerID=8YFLogxK
U2 - 10.1016/S0958-1669(02)00337-3
DO - 10.1016/S0958-1669(02)00337-3
M3 - Review article
C2 - 12323361
AN - SCOPUS:0036669618
SN - 0958-1669
VL - 13
SP - 376
EP - 384
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 4
ER -