Enzymatic hydrolysis of sphingolipids VII. Hydrolysis of gangliosides by a neuraminidase from calf brain

Zelina Leibovitz; Shimon Gatt

doi:10.1016/0005-2760(68)90015-5

Enzymatic hydrolysis of sphingolipids VII. Hydrolysis of gangliosides by a neuraminidase from calf brain

Zelina Leibovitz, Shimon Gatt

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Abstract

A neuraminidase has been partially purified by extracting calf brain acetone powder with Triton X-100. It has an optimal pH at 4.4 and hydrolyzed tri- and disialogangliosides as well as "hematoside". It did not hydrolyze monosialoganglioside, sialyllactose nor a sialic acid-containing glycoprotein. The sialic acid residue of "Tay-Sachs' ganglioside" could be split off only after previous treatment with β-N-acetylhexosaminidase. A pathway for the total degradation of brain gangliosides by the neuraminidase and four other brain enzymes is presented.

Original language	English
Pages (from-to)	136-143
Number of pages	8
Journal	Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume	152
Issue number	1
DOIs	https://doi.org/10.1016/0005-2760(68)90015-5
State	Published - 10 Jan 1968
Externally published	Yes

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title = "Enzymatic hydrolysis of sphingolipids VII. Hydrolysis of gangliosides by a neuraminidase from calf brain",

abstract = "A neuraminidase has been partially purified by extracting calf brain acetone powder with Triton X-100. It has an optimal pH at 4.4 and hydrolyzed tri- and disialogangliosides as well as {"}hematoside{"}. It did not hydrolyze monosialoganglioside, sialyllactose nor a sialic acid-containing glycoprotein. The sialic acid residue of {"}Tay-Sachs' ganglioside{"} could be split off only after previous treatment with β-N-acetylhexosaminidase. A pathway for the total degradation of brain gangliosides by the neuraminidase and four other brain enzymes is presented.",

author = "Zelina Leibovitz and Shimon Gatt",

note = "Funding Information: We wish to thank Drs. H. WIEGANDT and R. M. MCCLUER for generous gifts of purified gangliosides and Dr. A. GAUHE for a sample of sialyllactose. This work was supported in part by a U.S. Public Health Service Research Grant (NBozg67).",

year = "1968",

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doi = "10.1016/0005-2760(68)90015-5",

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T1 - Enzymatic hydrolysis of sphingolipids VII. Hydrolysis of gangliosides by a neuraminidase from calf brain

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AU - Gatt, Shimon

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PY - 1968/1/10

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N2 - A neuraminidase has been partially purified by extracting calf brain acetone powder with Triton X-100. It has an optimal pH at 4.4 and hydrolyzed tri- and disialogangliosides as well as "hematoside". It did not hydrolyze monosialoganglioside, sialyllactose nor a sialic acid-containing glycoprotein. The sialic acid residue of "Tay-Sachs' ganglioside" could be split off only after previous treatment with β-N-acetylhexosaminidase. A pathway for the total degradation of brain gangliosides by the neuraminidase and four other brain enzymes is presented.

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ER -

Enzymatic hydrolysis of sphingolipids VII. Hydrolysis of gangliosides by a neuraminidase from calf brain

Abstract

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