Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique

J. H. Gu; H. Lu; Y. W. Chen; L. Y. Liu; P. Wang; J. M. Ma; Z. H. Lu

doi:10.1016/S0968-5677(98)00105-9

Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique

J. H. Gu, H. Lu, Y. W. Chen, L. Y. Liu, P. Wang, J. M. Ma, Z. H. Lu

Research output: Contribution to journal › Conference article › peer-review

28 Scopus citations

Abstract

In our experiment, colloidal gold was used to enhance the signal obtained from the surface-plasmon-resonance biosensor. Fab' fragments of human IgG molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300.

Original language	English
Pages (from-to)	695-698
Number of pages	4
Journal	Supramolecular Science
Volume	5
Issue number	5-6
DOIs	https://doi.org/10.1016/S0968-5677(98)00105-9
State	Published - 1998
Externally published	Yes
Event	Proceedings of the 7th International Conference on Molecular Electronics and Biocomputing - Nanjing, China Duration: 10 Nov 1997 → 12 Nov 1997

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title = "Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique",

abstract = "In our experiment, colloidal gold was used to enhance the signal obtained from the surface-plasmon-resonance biosensor. Fab' fragments of human IgG molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300.",

author = "Gu, {J. H.} and H. Lu and Chen, {Y. W.} and Liu, {L. Y.} and P. Wang and Ma, {J. M.} and Lu, {Z. H.}",

note = "Funding Information: One of the authors (J.H. Gu) would like to acknowledge the very useful communication with Prof. P.T. Leung of Portland State University of the United States of American and Prof. Gordon of the Amersham Laboratories (UK). This work was supported by the Chinese Nature Foundation (No. 39400033).; Proceedings of the 7th International Conference on Molecular Electronics and Biocomputing ; Conference date: 10-11-1997 Through 12-11-1997",

year = "1998",

doi = "10.1016/S0968-5677(98)00105-9",

language = "English",

volume = "5",

pages = "695--698",

journal = "Supramolecular Science",

issn = "0968-5677",

publisher = "Elsevier B.V.",

number = "5-6",

}

TY - JOUR

T1 - Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique

AU - Gu, J. H.

AU - Lu, H.

AU - Chen, Y. W.

AU - Liu, L. Y.

AU - Wang, P.

AU - Ma, J. M.

AU - Lu, Z. H.

N1 - Funding Information: One of the authors (J.H. Gu) would like to acknowledge the very useful communication with Prof. P.T. Leung of Portland State University of the United States of American and Prof. Gordon of the Amersham Laboratories (UK). This work was supported by the Chinese Nature Foundation (No. 39400033).

PY - 1998

Y1 - 1998

N2 - In our experiment, colloidal gold was used to enhance the signal obtained from the surface-plasmon-resonance biosensor. Fab' fragments of human IgG molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300.

AB - In our experiment, colloidal gold was used to enhance the signal obtained from the surface-plasmon-resonance biosensor. Fab' fragments of human IgG molecules were immobilized on the gold surface and reacted with the sheep-anti-human IgG(SAH-IgG) molecules in solution. It was found that when the colloidal gold labeling technique was introduced the sensitivity of surface plasmon resonance instruments increased by a factor of about 300.

UR - http://www.scopus.com/inward/record.url?scp=0032179136&partnerID=8YFLogxK

U2 - 10.1016/S0968-5677(98)00105-9

DO - 10.1016/S0968-5677(98)00105-9

M3 - Conference article

AN - SCOPUS:0032179136

SN - 0968-5677

VL - 5

SP - 695

EP - 698

JO - Supramolecular Science

JF - Supramolecular Science

IS - 5-6

T2 - Proceedings of the 7th International Conference on Molecular Electronics and Biocomputing

Y2 - 10 November 1997 through 12 November 1997

ER -

Enhancement of the sensitivity of surface plasmon resonance biosensor with colloidal gold labeling technique

Abstract

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