Enhanced production of IL-8 in chronic but not in early ileal lesions of Crohn's disease (CD)

E. Brandt, J. F. Colombel, N. Ectors, L. Gambiez, D. Emilie, K. Geboes, M. Capron, P. Desreumaux

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Distinct Th1/Th2 patterns have been observed during the evolution of CD. The aim of this study was to compare neutrophil involvement and IL-8 mRNA and protein expression during early recurrent lesions and chronic phases of CD. Twenty-nine patients with CD having ileocolonic resection with anastomosis were studied. Biopsies were obtained during surgery from the non-inflamed ileal mucosa and from chronic ileal lesions. Endoscopic ileal biopsies were also taken from early recurrent ileal lesions occurring 3 months after surgery. Neutrophil counts were performed and mucosal IL-8 levels were evaluated by competitive reverse transcriptase-polymerase chain reaction and immunohistochemistry. Early recurrent ileal lesions were characterized by low neutrophil counts and IL-8 production at the mRNA and protein levels compared with the ileal chronic lesions. The main cellular sources of IL-8 in the early recurrent lesions were neutrophils, while in chronic lesions the majority of IL-8-stained cells were CD3+ T cells and macrophages. These results confirmed that the nature of the inflammatory infiltrate and the expression of cytokine profiles may differ between the acute and chronic phases of CD.

Original languageEnglish
Pages (from-to)180-185
Number of pages6
JournalClinical and Experimental Immunology
Issue number2
StatePublished - 2000
Externally publishedYes


  • Chronic lesions
  • Crohn's disease
  • Early recurrent lesions
  • IL-8
  • Neutrophils


Dive into the research topics of 'Enhanced production of IL-8 in chronic but not in early ileal lesions of Crohn's disease (CD)'. Together they form a unique fingerprint.

Cite this