TY - JOUR
T1 - Enhanced gastric emptying of a liquid gastric load in mice lacking cholecystokinin-B receptor
T2 - A study of CCK-A,B, and AB receptor gene knockout mice
AU - Miyasaka, Kyoko
AU - Ohta, Minoru
AU - Kanai, Setsuko
AU - Yoshida, Yuki
AU - Sato, Norikazu
AU - Nagata, Aki
AU - Matsui, Toshimitsu
AU - Noda, Tetsuo
AU - Jimi, Atsuo
AU - Takiguchi, Soichi
AU - Takata, Yutaka
AU - Kawanami, Takako
AU - Funakoshi, Akihiro
PY - 2004/4
Y1 - 2004/4
N2 - Background. Although cholecystokinin (CCK) has been shown to inhibit gastric emptying via CCK-A receptors (CCK-ARs), the role of CCK-B receptors (CCK-BRs) has not been verified. We examined whether gastric emptying of a nonnutrient liquid load was modified in CCK-AR, BR, and ARBR gene knock-out mice. Methods. A liquid gastric load prepared with phenol red was administered via an orogastric tube (0.15ml/mouse). The animals were killed by decapitation, and gastric emptying was estimated at 10 and 30min after ingestion. The effects of the sulfated form of CCK-8 (CCK-8S) and of graded doses of atropine were examined. In addition, a proton pump inhibitor was administered to wild-type mice to examine the contribution of gastric acid to emptying. Results. Gastric emptying was significantly enhanced in mice lacking CCK-BR, as compared with wild-type and CCK-AR(-/-) mice. CCK-8S inhibited gastric emptying in mice with CCK-AR, but not in mice without CCK-AR. A proton pump inhibitor did not affect gastric emptying. Atropine dose dependently inhibited gastric emptying in all genotypes. The thickness of smooth muscle was comparable for all genotypes. Conclusions. The gastric emptying of a nonnutrient liquid load was enhanced in mice without CCK-BR, although the precise mechanism is not known.
AB - Background. Although cholecystokinin (CCK) has been shown to inhibit gastric emptying via CCK-A receptors (CCK-ARs), the role of CCK-B receptors (CCK-BRs) has not been verified. We examined whether gastric emptying of a nonnutrient liquid load was modified in CCK-AR, BR, and ARBR gene knock-out mice. Methods. A liquid gastric load prepared with phenol red was administered via an orogastric tube (0.15ml/mouse). The animals were killed by decapitation, and gastric emptying was estimated at 10 and 30min after ingestion. The effects of the sulfated form of CCK-8 (CCK-8S) and of graded doses of atropine were examined. In addition, a proton pump inhibitor was administered to wild-type mice to examine the contribution of gastric acid to emptying. Results. Gastric emptying was significantly enhanced in mice lacking CCK-BR, as compared with wild-type and CCK-AR(-/-) mice. CCK-8S inhibited gastric emptying in mice with CCK-AR, but not in mice without CCK-AR. A proton pump inhibitor did not affect gastric emptying. Atropine dose dependently inhibited gastric emptying in all genotypes. The thickness of smooth muscle was comparable for all genotypes. Conclusions. The gastric emptying of a nonnutrient liquid load was enhanced in mice without CCK-BR, although the precise mechanism is not known.
KW - CCK
KW - CCK-B receptor
KW - Gastric acid
KW - Gastric emptying
UR - http://www.scopus.com/inward/record.url?scp=2542438864&partnerID=8YFLogxK
U2 - 10.1007/s00535-003-1297-2
DO - 10.1007/s00535-003-1297-2
M3 - Article
C2 - 15168241
AN - SCOPUS:2542438864
SN - 0944-1174
VL - 39
SP - 319
EP - 323
JO - Journal of Gastroenterology
JF - Journal of Gastroenterology
IS - 4
ER -