Engineering A11 minibody-conjugated, polypeptide-based gold nanoshells for prostate stem cell antigen (PSCA)– targeted photothermal therapy

Kristine M. Mayle, Kathryn R. Dern, Vincent K. Wong, Kevin Y. Chen, Shijun Sung, Ke Ding, April R. Rodriguez, Scott Knowles, Zachary Taylor, Z. Hong Zhou, Warren S. Grundfest, Anna M. Wu, Timothy J. Deming, Daniel T. Kamei

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Currently, there is no curative treatment for advanced metastatic prostate cancer, and options, such as chemotherapy, are often nonspecific, harming healthy cells and resulting in severe side effects. Attaching targeting ligands to agents used in anticancer therapies has been shown to improve efficacy and reduce nonspecific toxicity. Furthermore, the use of triggered therapies can enable spatial and temporal control over the treatment. Here, we combined an engineered prostate cancer–specific targeting ligand, the A11 minibody, with a novel photothermal therapy agent, polypeptide-based gold nanoshells, which generate heat in response to near-infrared light. We show that the A11 minibody strongly binds to the prostate stem cell antigen that is overexpressed on the surface of metastatic prostate cancer cells. Compared to nonconjugated gold nanoshells, our A11 minibody-conjugated gold nanoshell exhibited significant laser-induced, localized killing of prostate cancer cells in vitro. In addition, we improved upon a comprehensive heat transfer mathematical model that was previously developed by our laboratory. By relaxing some of the assumptions of our earlier model, we were able to generate more accurate predictions for this particular study. Our experimental and theoretical results demonstrate the potential of our novel minibody-conjugated gold nanoshells for metastatic prostate cancer therapy.

Original languageEnglish
Pages (from-to)26-35
Number of pages10
JournalSLAS Technology
Volume22
Issue number1
DOIs
StatePublished - Feb 2017
Externally publishedYes

Keywords

  • Gold nanoshells
  • Photothermal therapy
  • Polypeptide vesicles

Fingerprint

Dive into the research topics of 'Engineering A11 minibody-conjugated, polypeptide-based gold nanoshells for prostate stem cell antigen (PSCA)– targeted photothermal therapy'. Together they form a unique fingerprint.

Cite this